Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus

The triple-gene-block protein 2 (TGBp2) of Bamboo mosaic virus (BaMV) is a transmembrane protein which was proposed to be involved in viral RNA binding during virus transport. Here, we report on the RNA-binding properties of TGBp2. Using tyrosine fluorescence spectroscopy and UV-crosslinking assays,...

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Autores principales: Hsu, Hsiu-Ting, Tseng, Yang-Hao, Chou, Yuan-Lin, Su, Shiaw-Hwa, Hsu, Yau-Heiu, Chang, Ban-Yang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2689192/
https://www.ncbi.nlm.nih.gov/pubmed/19422690
http://dx.doi.org/10.1186/1743-422X-6-50
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author Hsu, Hsiu-Ting
Tseng, Yang-Hao
Chou, Yuan-Lin
Su, Shiaw-Hwa
Hsu, Yau-Heiu
Chang, Ban-Yang
author_facet Hsu, Hsiu-Ting
Tseng, Yang-Hao
Chou, Yuan-Lin
Su, Shiaw-Hwa
Hsu, Yau-Heiu
Chang, Ban-Yang
author_sort Hsu, Hsiu-Ting
collection PubMed
description The triple-gene-block protein 2 (TGBp2) of Bamboo mosaic virus (BaMV) is a transmembrane protein which was proposed to be involved in viral RNA binding during virus transport. Here, we report on the RNA-binding properties of TGBp2. Using tyrosine fluorescence spectroscopy and UV-crosslinking assays, the TGBp2 solubilized with Triton X-100 was found to interact with viral RNA in a non-specific manner. These results raise the possibility that TGBp2 facilitates intracellular delivery of viral RNA through non-specific protein-RNA interaction.
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spelling pubmed-26891922009-06-02 Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus Hsu, Hsiu-Ting Tseng, Yang-Hao Chou, Yuan-Lin Su, Shiaw-Hwa Hsu, Yau-Heiu Chang, Ban-Yang Virol J Short Report The triple-gene-block protein 2 (TGBp2) of Bamboo mosaic virus (BaMV) is a transmembrane protein which was proposed to be involved in viral RNA binding during virus transport. Here, we report on the RNA-binding properties of TGBp2. Using tyrosine fluorescence spectroscopy and UV-crosslinking assays, the TGBp2 solubilized with Triton X-100 was found to interact with viral RNA in a non-specific manner. These results raise the possibility that TGBp2 facilitates intracellular delivery of viral RNA through non-specific protein-RNA interaction. BioMed Central 2009-05-07 /pmc/articles/PMC2689192/ /pubmed/19422690 http://dx.doi.org/10.1186/1743-422X-6-50 Text en Copyright © 2009 Hsu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Hsu, Hsiu-Ting
Tseng, Yang-Hao
Chou, Yuan-Lin
Su, Shiaw-Hwa
Hsu, Yau-Heiu
Chang, Ban-Yang
Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus
title Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus
title_full Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus
title_fullStr Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus
title_full_unstemmed Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus
title_short Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus
title_sort characterization of the rna-binding properties of the triple-gene-block protein 2 of bamboo mosaic virus
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2689192/
https://www.ncbi.nlm.nih.gov/pubmed/19422690
http://dx.doi.org/10.1186/1743-422X-6-50
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