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Molecular beacon-based real-time PCR detection of primary isolates of Salmonella Typhimurium and Salmonella Enteritidis in environmental and clinical samples

BACKGROUND: A fast and simple two-step multiplex real-time PCR assay has been developed to replace the traditional, laborious Salmonella serotyping procedure. Molecular beacons were incorporated into the assay as probes for target DNA. Target sequences were regions of the invA, prot6E and fliC genes...

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Detalles Bibliográficos
Autores principales:	Hadjinicolaou, Andreas V, Demetriou, Victoria L, Emmanuel, Maria A, Kakoyiannis, Charalambos K, Kostrikis, Leondios G
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Methodology article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2689230/ https://www.ncbi.nlm.nih.gov/pubmed/19454003 http://dx.doi.org/10.1186/1471-2180-9-97

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