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Bergmann Glia and the Recognition Molecule CHL1 Organize GABAergic Axons and Direct Innervation of Purkinje Cell Dendrites

The geometric and subcellular organization of axon arbors distributes and regulates electrical signaling in neurons and networks, but the underlying mechanisms have remained elusive. In rodent cerebellar cortex, stellate interneurons elaborate characteristic axon arbors that selectively innervate Pu...

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Autores principales: Ango, Fabrice, Wu, Caizhi, Van der Want, Johannes J., Wu, Priscilla, Schachner, Melitta, Huang, Z. Josh
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2689695/
https://www.ncbi.nlm.nih.gov/pubmed/18447583
http://dx.doi.org/10.1371/journal.pbio.0060103
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author Ango, Fabrice
Wu, Caizhi
Van der Want, Johannes J.
Wu, Priscilla
Schachner, Melitta
Huang, Z. Josh
author_facet Ango, Fabrice
Wu, Caizhi
Van der Want, Johannes J.
Wu, Priscilla
Schachner, Melitta
Huang, Z. Josh
author_sort Ango, Fabrice
collection PubMed
description The geometric and subcellular organization of axon arbors distributes and regulates electrical signaling in neurons and networks, but the underlying mechanisms have remained elusive. In rodent cerebellar cortex, stellate interneurons elaborate characteristic axon arbors that selectively innervate Purkinje cell dendrites and likely regulate dendritic integration. We used GFP BAC transgenic reporter mice to examine the cellular processes and molecular mechanisms underlying the development of stellate cell axons and their innervation pattern. We show that stellate axons are organized and guided towards Purkinje cell dendrites by an intermediate scaffold of Bergmann glial (BG) fibers. The L1 family immunoglobulin protein Close Homologue of L1 (CHL1) is localized to apical BG fibers and stellate cells during the development of stellate axon arbors. In the absence of CHL1, stellate axons deviate from BG fibers and show aberrant branching and orientation. Furthermore, synapse formation between aberrant stellate axons and Purkinje dendrites is reduced and cannot be maintained, leading to progressive atrophy of axon terminals. These results establish BG fibers as a guiding scaffold and CHL1 a molecular signal in the organization of stellate axon arbors and in directing their dendritic innervation.
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spelling pubmed-26896952009-06-02 Bergmann Glia and the Recognition Molecule CHL1 Organize GABAergic Axons and Direct Innervation of Purkinje Cell Dendrites Ango, Fabrice Wu, Caizhi Van der Want, Johannes J. Wu, Priscilla Schachner, Melitta Huang, Z. Josh PLoS Biol Research Article The geometric and subcellular organization of axon arbors distributes and regulates electrical signaling in neurons and networks, but the underlying mechanisms have remained elusive. In rodent cerebellar cortex, stellate interneurons elaborate characteristic axon arbors that selectively innervate Purkinje cell dendrites and likely regulate dendritic integration. We used GFP BAC transgenic reporter mice to examine the cellular processes and molecular mechanisms underlying the development of stellate cell axons and their innervation pattern. We show that stellate axons are organized and guided towards Purkinje cell dendrites by an intermediate scaffold of Bergmann glial (BG) fibers. The L1 family immunoglobulin protein Close Homologue of L1 (CHL1) is localized to apical BG fibers and stellate cells during the development of stellate axon arbors. In the absence of CHL1, stellate axons deviate from BG fibers and show aberrant branching and orientation. Furthermore, synapse formation between aberrant stellate axons and Purkinje dendrites is reduced and cannot be maintained, leading to progressive atrophy of axon terminals. These results establish BG fibers as a guiding scaffold and CHL1 a molecular signal in the organization of stellate axon arbors and in directing their dendritic innervation. Public Library of Science 2008-04 2008-04-29 /pmc/articles/PMC2689695/ /pubmed/18447583 http://dx.doi.org/10.1371/journal.pbio.0060103 Text en © 2008 Ango et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ango, Fabrice
Wu, Caizhi
Van der Want, Johannes J.
Wu, Priscilla
Schachner, Melitta
Huang, Z. Josh
Bergmann Glia and the Recognition Molecule CHL1 Organize GABAergic Axons and Direct Innervation of Purkinje Cell Dendrites
title Bergmann Glia and the Recognition Molecule CHL1 Organize GABAergic Axons and Direct Innervation of Purkinje Cell Dendrites
title_full Bergmann Glia and the Recognition Molecule CHL1 Organize GABAergic Axons and Direct Innervation of Purkinje Cell Dendrites
title_fullStr Bergmann Glia and the Recognition Molecule CHL1 Organize GABAergic Axons and Direct Innervation of Purkinje Cell Dendrites
title_full_unstemmed Bergmann Glia and the Recognition Molecule CHL1 Organize GABAergic Axons and Direct Innervation of Purkinje Cell Dendrites
title_short Bergmann Glia and the Recognition Molecule CHL1 Organize GABAergic Axons and Direct Innervation of Purkinje Cell Dendrites
title_sort bergmann glia and the recognition molecule chl1 organize gabaergic axons and direct innervation of purkinje cell dendrites
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2689695/
https://www.ncbi.nlm.nih.gov/pubmed/18447583
http://dx.doi.org/10.1371/journal.pbio.0060103
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