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Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines

BACKGROUND: The immunological response to solid tumours is insufficient. Therefore, tumour specific antigens have been explored to facilitate the activation of the immune system. The cancer/testis antigen class of MAGE-A antigens is a possible target for vaccination. Their differential expression pr...

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Autores principales: Müller-Richter, Urs DA, Dowejko, Albert, Reuther, Tobias, Kleinheinz, Johannes, Reichert, Torsten E, Driemel, Oliver
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2690579/
https://www.ncbi.nlm.nih.gov/pubmed/19358718
http://dx.doi.org/10.1186/1746-160X-5-10
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author Müller-Richter, Urs DA
Dowejko, Albert
Reuther, Tobias
Kleinheinz, Johannes
Reichert, Torsten E
Driemel, Oliver
author_facet Müller-Richter, Urs DA
Dowejko, Albert
Reuther, Tobias
Kleinheinz, Johannes
Reichert, Torsten E
Driemel, Oliver
author_sort Müller-Richter, Urs DA
collection PubMed
description BACKGROUND: The immunological response to solid tumours is insufficient. Therefore, tumour specific antigens have been explored to facilitate the activation of the immune system. The cancer/testis antigen class of MAGE-A antigens is a possible target for vaccination. Their differential expression profiles also modulate the course of the cancer disease and its response to antineoplastic drugs. METHODS: The expression profiles of MAGE-A2, -A3, -A4, -A6 and -A10 in five own oral squamous cell carcinoma cell lines were characterised by rt-PCR, qrt-PCR and immunocytochemistry with a global MAGE-A antibody (57B) and compared with those of an adult keratinocyte cell line (NHEK). RESULTS: All tumour cell lines expressed MAGE-A antigens. The antigens were expressed in groups with different preferences. The predominant antigens expressed were MAGE-A2, -A3 and -A6. MAGE-A10 was not expressed in the cell lines tested. The MAGE-A gene products detected in the adult keratinocyte cell line NHEK were used as a reference. CONCLUSION: MAGE-A antigens are expressed in oral squamous cell carcinomas. The expression profiles measured facilitate distinct examinations in forthcoming studies on responses to antineoplastic drugs or radiation therapy. MAGE-A antigens are still an interesting aim for immunotherapy.
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spelling pubmed-26905792009-06-04 Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines Müller-Richter, Urs DA Dowejko, Albert Reuther, Tobias Kleinheinz, Johannes Reichert, Torsten E Driemel, Oliver Head Face Med Research BACKGROUND: The immunological response to solid tumours is insufficient. Therefore, tumour specific antigens have been explored to facilitate the activation of the immune system. The cancer/testis antigen class of MAGE-A antigens is a possible target for vaccination. Their differential expression profiles also modulate the course of the cancer disease and its response to antineoplastic drugs. METHODS: The expression profiles of MAGE-A2, -A3, -A4, -A6 and -A10 in five own oral squamous cell carcinoma cell lines were characterised by rt-PCR, qrt-PCR and immunocytochemistry with a global MAGE-A antibody (57B) and compared with those of an adult keratinocyte cell line (NHEK). RESULTS: All tumour cell lines expressed MAGE-A antigens. The antigens were expressed in groups with different preferences. The predominant antigens expressed were MAGE-A2, -A3 and -A6. MAGE-A10 was not expressed in the cell lines tested. The MAGE-A gene products detected in the adult keratinocyte cell line NHEK were used as a reference. CONCLUSION: MAGE-A antigens are expressed in oral squamous cell carcinomas. The expression profiles measured facilitate distinct examinations in forthcoming studies on responses to antineoplastic drugs or radiation therapy. MAGE-A antigens are still an interesting aim for immunotherapy. BioMed Central 2009-04-09 /pmc/articles/PMC2690579/ /pubmed/19358718 http://dx.doi.org/10.1186/1746-160X-5-10 Text en Copyright © 2009 Müller-Richter et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Müller-Richter, Urs DA
Dowejko, Albert
Reuther, Tobias
Kleinheinz, Johannes
Reichert, Torsten E
Driemel, Oliver
Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines
title Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines
title_full Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines
title_fullStr Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines
title_full_unstemmed Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines
title_short Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines
title_sort analysis of expression profiles of mage-a antigens in oral squamous cell carcinoma cell lines
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2690579/
https://www.ncbi.nlm.nih.gov/pubmed/19358718
http://dx.doi.org/10.1186/1746-160X-5-10
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