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HCO(3)(−)-dependent volume regulation in α-cells of the rat endocrine pancreas
Ion transport activity in pancreatic α-cells was assessed by studying cell volume regulation in response to anisotonic solutions. Cell volume was measured by a video imaging method, and cells were superfused with either 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid-buffered or HCO(3)(−)-buffere...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer-Verlag
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691524/ https://www.ncbi.nlm.nih.gov/pubmed/19214560 http://dx.doi.org/10.1007/s00424-009-0644-4 |
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author | Davies, Sarah L. Best, Len Brown, Peter D. |
author_facet | Davies, Sarah L. Best, Len Brown, Peter D. |
author_sort | Davies, Sarah L. |
collection | PubMed |
description | Ion transport activity in pancreatic α-cells was assessed by studying cell volume regulation in response to anisotonic solutions. Cell volume was measured by a video imaging method, and cells were superfused with either 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid-buffered or HCO(3)(−)-buffered solutions. α-Cells did not exhibit a regulatory volume increase (RVI) in response to cell shrinkage caused by hypertonic solutions. A RVI was observed, however, in cells that had first undergone a regulatory volume decrease (RVD), but only in HCO(3)(−)-buffered solutions. RVI was also observed in response to a HCO(3)(−)-buffered hypertonic solution in which the glucose concentration was increased from 4 to 20 mM. The post-RVD RVI and the glucose-induced RVI were both inhibited by 10 μM 5-(N-methyl-N-isobutyl) amiloride or 100 μM 2,2′-(1,2-ethenediyl) bis (5-isothio-cyanatobenzenesulfonic acid), but not by 10 μM benzamil nor 10 μM bumetanide. These data suggest that Na(+)–H(+) exchangers and Cl(−)–HCO(3)(−) exchangers contribute to volume regulation in α-cells. |
format | Text |
id | pubmed-2691524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-26915242009-06-05 HCO(3)(−)-dependent volume regulation in α-cells of the rat endocrine pancreas Davies, Sarah L. Best, Len Brown, Peter D. Pflugers Arch Transport Physiology Ion transport activity in pancreatic α-cells was assessed by studying cell volume regulation in response to anisotonic solutions. Cell volume was measured by a video imaging method, and cells were superfused with either 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid-buffered or HCO(3)(−)-buffered solutions. α-Cells did not exhibit a regulatory volume increase (RVI) in response to cell shrinkage caused by hypertonic solutions. A RVI was observed, however, in cells that had first undergone a regulatory volume decrease (RVD), but only in HCO(3)(−)-buffered solutions. RVI was also observed in response to a HCO(3)(−)-buffered hypertonic solution in which the glucose concentration was increased from 4 to 20 mM. The post-RVD RVI and the glucose-induced RVI were both inhibited by 10 μM 5-(N-methyl-N-isobutyl) amiloride or 100 μM 2,2′-(1,2-ethenediyl) bis (5-isothio-cyanatobenzenesulfonic acid), but not by 10 μM benzamil nor 10 μM bumetanide. These data suggest that Na(+)–H(+) exchangers and Cl(−)–HCO(3)(−) exchangers contribute to volume regulation in α-cells. Springer-Verlag 2009-02-13 2009-07 /pmc/articles/PMC2691524/ /pubmed/19214560 http://dx.doi.org/10.1007/s00424-009-0644-4 Text en © The Author(s) 2009 |
spellingShingle | Transport Physiology Davies, Sarah L. Best, Len Brown, Peter D. HCO(3)(−)-dependent volume regulation in α-cells of the rat endocrine pancreas |
title | HCO(3)(−)-dependent volume regulation in α-cells of the rat endocrine pancreas |
title_full | HCO(3)(−)-dependent volume regulation in α-cells of the rat endocrine pancreas |
title_fullStr | HCO(3)(−)-dependent volume regulation in α-cells of the rat endocrine pancreas |
title_full_unstemmed | HCO(3)(−)-dependent volume regulation in α-cells of the rat endocrine pancreas |
title_short | HCO(3)(−)-dependent volume regulation in α-cells of the rat endocrine pancreas |
title_sort | hco(3)(−)-dependent volume regulation in α-cells of the rat endocrine pancreas |
topic | Transport Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691524/ https://www.ncbi.nlm.nih.gov/pubmed/19214560 http://dx.doi.org/10.1007/s00424-009-0644-4 |
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