Gene Expression Profiling as a Tool for Positional Cloning of Genes-Shortcut or the Longest Way Round

The identification of quantitative trait loci, QTL, in arthritis animal models is a straight forward process. However, to identify the underlying genes is a great challenge. One strategy frequently used, is to combine QTL analysis with genomic/proteomic screens. This has resulted in a number of publications where carefully performed genomic analyses present likely candidate genes for their respective QTL´s. However, seldom the findings are reconnected to the QTL controlled phenotypes. In this review, we use our own data as an illustrative example that “very likely candidate genes” identified by genomic/proteomics is not necessarily the same as true QTL underlying genes.