Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs

We developed a simple, direct and cost-effective approach to search for the most likely target genes of a known microRNA (miRNA) in vitro. We term this method ‘labeled miRNA pull-down (LAMP)’ assay system. Briefly, the pre-miRNA is labeled with digoxigenin (DIG), mixed with cell extracts and immunop...

Descripción completa

Detalles Bibliográficos
Autores principales: Hsu, Ren-Jun, Yang, Hsin-Jung, Tsai, Huai-Jen
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691847/
https://www.ncbi.nlm.nih.gov/pubmed/19420057
http://dx.doi.org/10.1093/nar/gkp274

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691847/
https://www.ncbi.nlm.nih.gov/pubmed/19420057
http://dx.doi.org/10.1093/nar/gkp274

Ejemplares similares