Cargando…
Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs
We developed a simple, direct and cost-effective approach to search for the most likely target genes of a known microRNA (miRNA) in vitro. We term this method ‘labeled miRNA pull-down (LAMP)’ assay system. Briefly, the pre-miRNA is labeled with digoxigenin (DIG), mixed with cell extracts and immunop...
| Autores principales: | , , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2009
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2691847/ https://www.ncbi.nlm.nih.gov/pubmed/19420057 http://dx.doi.org/10.1093/nar/gkp274 |