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Complementary regulation of four Eucalyptus CBF genes under various cold conditions

CBF transcription factors play central roles in the control of freezing tolerance in plants. The isolation of two additional CBF genes, EguCBF1c and EguCBF1d, from E. gunnii, one of the cold-hardiest Eucalyptus species, is described. While the EguCBF1D protein sequence is very similar to the previou...

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Autores principales: Navarro, M., Marque, G., Ayax, C., Keller, G., Borges, J. P., Marque, C., Teulières, C.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2692017/
https://www.ncbi.nlm.nih.gov/pubmed/19457981
http://dx.doi.org/10.1093/jxb/erp129
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author Navarro, M.
Marque, G.
Ayax, C.
Keller, G.
Borges, J. P.
Marque, C.
Teulières, C.
author_facet Navarro, M.
Marque, G.
Ayax, C.
Keller, G.
Borges, J. P.
Marque, C.
Teulières, C.
author_sort Navarro, M.
collection PubMed
description CBF transcription factors play central roles in the control of freezing tolerance in plants. The isolation of two additional CBF genes, EguCBF1c and EguCBF1d, from E. gunnii, one of the cold-hardiest Eucalyptus species, is described. While the EguCBF1D protein sequence is very similar to the previously characterized EguCBF1A and EguCBF1B sequences, EguCBF1C is more distinctive, in particular in the AP2-DBD (AP2-DNA binding domain). The expression analysis of the four genes by RT-qPCR reveals that none of them is specific to one stress but they are all preferentially induced by cold, except for the EguCBF1c gene which is more responsive to salt. The calculation of the transcript copy number enables the quantification of constitutive CBF gene expression. This basal level, significant for the four genes, greatly influences the final EguCBF1 transcript level in the cold. A cold shock at 4 °C, as well as a progressive freezing which mimics a natural frost episode, trigger a fast and strong response of the EguCBF1 genes, while growth at acclimating temperatures results in a lower but more durable induction. The differential expression of the four EguCBF1 genes under these cold regimes suggests that there is a complementary regulation. The high accumulation of the CBF transcript, observed in response to the different types of cold conditions, might be a key for the winter survival of this evergreen broad-leaved tree.
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spelling pubmed-26920172009-06-09 Complementary regulation of four Eucalyptus CBF genes under various cold conditions Navarro, M. Marque, G. Ayax, C. Keller, G. Borges, J. P. Marque, C. Teulières, C. J Exp Bot Research Papers CBF transcription factors play central roles in the control of freezing tolerance in plants. The isolation of two additional CBF genes, EguCBF1c and EguCBF1d, from E. gunnii, one of the cold-hardiest Eucalyptus species, is described. While the EguCBF1D protein sequence is very similar to the previously characterized EguCBF1A and EguCBF1B sequences, EguCBF1C is more distinctive, in particular in the AP2-DBD (AP2-DNA binding domain). The expression analysis of the four genes by RT-qPCR reveals that none of them is specific to one stress but they are all preferentially induced by cold, except for the EguCBF1c gene which is more responsive to salt. The calculation of the transcript copy number enables the quantification of constitutive CBF gene expression. This basal level, significant for the four genes, greatly influences the final EguCBF1 transcript level in the cold. A cold shock at 4 °C, as well as a progressive freezing which mimics a natural frost episode, trigger a fast and strong response of the EguCBF1 genes, while growth at acclimating temperatures results in a lower but more durable induction. The differential expression of the four EguCBF1 genes under these cold regimes suggests that there is a complementary regulation. The high accumulation of the CBF transcript, observed in response to the different types of cold conditions, might be a key for the winter survival of this evergreen broad-leaved tree. Oxford University Press 2009-07 2009-05-20 /pmc/articles/PMC2692017/ /pubmed/19457981 http://dx.doi.org/10.1093/jxb/erp129 Text en © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Navarro, M.
Marque, G.
Ayax, C.
Keller, G.
Borges, J. P.
Marque, C.
Teulières, C.
Complementary regulation of four Eucalyptus CBF genes under various cold conditions
title Complementary regulation of four Eucalyptus CBF genes under various cold conditions
title_full Complementary regulation of four Eucalyptus CBF genes under various cold conditions
title_fullStr Complementary regulation of four Eucalyptus CBF genes under various cold conditions
title_full_unstemmed Complementary regulation of four Eucalyptus CBF genes under various cold conditions
title_short Complementary regulation of four Eucalyptus CBF genes under various cold conditions
title_sort complementary regulation of four eucalyptus cbf genes under various cold conditions
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2692017/
https://www.ncbi.nlm.nih.gov/pubmed/19457981
http://dx.doi.org/10.1093/jxb/erp129
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