Automated screening for mutants affecting dopaminergic neuron specification in C. elegans

We describe an automated method to isolate mutant C. elegans animals, which fail to appropriately execute a cellular differentiation program that we monitor with gfp-based reporter gene technology. A fluorescence activated sorting mechanism implemented in the COPAS Biosort machine is able to isolate...

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Detalles Bibliográficos
Autores principales: Doitsidou, Maria, Flames, Nuria, Lee, Albert C., Boyanov, Alexander, Hobert, Oliver
Formato: Texto
Lenguaje:English
Publicado: 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2693092/
https://www.ncbi.nlm.nih.gov/pubmed/18758453
http://dx.doi.org/10.1038/nmeth.1250
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author Doitsidou, Maria
Flames, Nuria
Lee, Albert C.
Boyanov, Alexander
Hobert, Oliver
author_facet Doitsidou, Maria
Flames, Nuria
Lee, Albert C.
Boyanov, Alexander
Hobert, Oliver
author_sort Doitsidou, Maria
collection PubMed
description We describe an automated method to isolate mutant C. elegans animals, which fail to appropriately execute a cellular differentiation program that we monitor with gfp-based reporter gene technology. A fluorescence activated sorting mechanism implemented in the COPAS Biosort machine is able to isolate mutants with subtle alterations in the cellular specificity of gfp expression. This methodology is significantly more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons fail to differentiate appropriately.
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spelling pubmed-26930922009-06-08 Automated screening for mutants affecting dopaminergic neuron specification in C. elegans Doitsidou, Maria Flames, Nuria Lee, Albert C. Boyanov, Alexander Hobert, Oliver Nat Methods Article We describe an automated method to isolate mutant C. elegans animals, which fail to appropriately execute a cellular differentiation program that we monitor with gfp-based reporter gene technology. A fluorescence activated sorting mechanism implemented in the COPAS Biosort machine is able to isolate mutants with subtle alterations in the cellular specificity of gfp expression. This methodology is significantly more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons fail to differentiate appropriately. 2008-08-31 2008-10 /pmc/articles/PMC2693092/ /pubmed/18758453 http://dx.doi.org/10.1038/nmeth.1250 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Doitsidou, Maria
Flames, Nuria
Lee, Albert C.
Boyanov, Alexander
Hobert, Oliver
Automated screening for mutants affecting dopaminergic neuron specification in C. elegans
title Automated screening for mutants affecting dopaminergic neuron specification in C. elegans
title_full Automated screening for mutants affecting dopaminergic neuron specification in C. elegans
title_fullStr Automated screening for mutants affecting dopaminergic neuron specification in C. elegans
title_full_unstemmed Automated screening for mutants affecting dopaminergic neuron specification in C. elegans
title_short Automated screening for mutants affecting dopaminergic neuron specification in C. elegans
title_sort automated screening for mutants affecting dopaminergic neuron specification in c. elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2693092/
https://www.ncbi.nlm.nih.gov/pubmed/18758453
http://dx.doi.org/10.1038/nmeth.1250
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