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Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies

The aim of this study was to ascertain the folate receptor (FR) targetability by an in vitro study and to acquire FR-targeted images in vivo models by using synthetic folate conjugates. PEG-folate was synthesized and labeled with (99m)Tc and fluorescein isothiocynate (FITC). Cell uptake studies were...

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Autores principales: Kim, Se-Lim, Jeong, Hwan-Jeong, Kim, Eun-Mi, Lee, Chang-Moon, Kwon, Tae-Hyoung, Sohn, Myung-Hee
Formato: Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2693629/
https://www.ncbi.nlm.nih.gov/pubmed/17596645
http://dx.doi.org/10.3346/jkms.2007.22.3.405
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author Kim, Se-Lim
Jeong, Hwan-Jeong
Kim, Eun-Mi
Lee, Chang-Moon
Kwon, Tae-Hyoung
Sohn, Myung-Hee
author_facet Kim, Se-Lim
Jeong, Hwan-Jeong
Kim, Eun-Mi
Lee, Chang-Moon
Kwon, Tae-Hyoung
Sohn, Myung-Hee
author_sort Kim, Se-Lim
collection PubMed
description The aim of this study was to ascertain the folate receptor (FR) targetability by an in vitro study and to acquire FR-targeted images in vivo models by using synthetic folate conjugates. PEG-folate was synthesized and labeled with (99m)Tc and fluorescein isothiocynate (FITC). Cell uptake studies were carried out in KB cells (FR-positive) and A549 cells (FR-negative) using FITC- and the (99m)Tc-labeled conjugates. The radiolabeled conjugate was intravenously injected to KB tumor xenografted mice. After it was injected, gamma images were recorded at 30 min, 1, 2, 3 and 4 hr. Cell uptake studies showed a difference between the KB cells and the A549 cells by flow cytometry analysis and gamma counting. On in vivo images, the tumor-to-normal muscle ratio was greater than 4. It ascertained that the PEG-folate conjugate specifically binds to the FR expressed on tumor cells in vitro. Moreover, it was possible to acquire the FR-targeted gamma images using PEG-folate conjugates in tumor models.
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spelling pubmed-26936292009-06-11 Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies Kim, Se-Lim Jeong, Hwan-Jeong Kim, Eun-Mi Lee, Chang-Moon Kwon, Tae-Hyoung Sohn, Myung-Hee J Korean Med Sci Original Article The aim of this study was to ascertain the folate receptor (FR) targetability by an in vitro study and to acquire FR-targeted images in vivo models by using synthetic folate conjugates. PEG-folate was synthesized and labeled with (99m)Tc and fluorescein isothiocynate (FITC). Cell uptake studies were carried out in KB cells (FR-positive) and A549 cells (FR-negative) using FITC- and the (99m)Tc-labeled conjugates. The radiolabeled conjugate was intravenously injected to KB tumor xenografted mice. After it was injected, gamma images were recorded at 30 min, 1, 2, 3 and 4 hr. Cell uptake studies showed a difference between the KB cells and the A549 cells by flow cytometry analysis and gamma counting. On in vivo images, the tumor-to-normal muscle ratio was greater than 4. It ascertained that the PEG-folate conjugate specifically binds to the FR expressed on tumor cells in vitro. Moreover, it was possible to acquire the FR-targeted gamma images using PEG-folate conjugates in tumor models. The Korean Academy of Medical Sciences 2007-06 2007-06-30 /pmc/articles/PMC2693629/ /pubmed/17596645 http://dx.doi.org/10.3346/jkms.2007.22.3.405 Text en Copyright © 2007 The Korean Academy of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Se-Lim
Jeong, Hwan-Jeong
Kim, Eun-Mi
Lee, Chang-Moon
Kwon, Tae-Hyoung
Sohn, Myung-Hee
Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies
title Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies
title_full Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies
title_fullStr Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies
title_full_unstemmed Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies
title_short Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies
title_sort folate receptor targeted imaging using poly (ethylene glycol)-folate: in vitro and in vivo studies
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2693629/
https://www.ncbi.nlm.nih.gov/pubmed/17596645
http://dx.doi.org/10.3346/jkms.2007.22.3.405
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