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Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4(+ )T cells in sarcoidosis
BACKGROUND: Activated T helper (Th)-1 pulmonary CD4(+ )cells and their mediators are essential for the inflammation and granulomatous process in sarcoidosis. Recently, T-cell immunoglobulin and mucin domain (TIM) molecules were suggested to be important regulators of immune function. In this study,...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2694180/ https://www.ncbi.nlm.nih.gov/pubmed/19480659 http://dx.doi.org/10.1186/1465-9921-10-42 |
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author | Idali, Farah Wahlström, Jan Dahlberg, Benita Khademi, Mohsen Olsson, Tomas Eklund, Anders Grunewald, Johan |
author_facet | Idali, Farah Wahlström, Jan Dahlberg, Benita Khademi, Mohsen Olsson, Tomas Eklund, Anders Grunewald, Johan |
author_sort | Idali, Farah |
collection | PubMed |
description | BACKGROUND: Activated T helper (Th)-1 pulmonary CD4(+ )cells and their mediators are essential for the inflammation and granulomatous process in sarcoidosis. Recently, T-cell immunoglobulin and mucin domain (TIM) molecules were suggested to be important regulators of immune function. In this study, we wanted to investigate whether TIM molecules could play a role in sarcoidosis. METHODS: We used real-time polymerase chain reaction to investigate the differential gene expression of TIM-1 and TIM-3 as well as a few Th1 and Th2 cytokines (IL-2, IFN-γ, IL-4, IL-5 and IL-13) in CD4(+ )T cells isolated from bronchoalveolar lavage fluid (BALF) of patients (n = 28) and healthy controls (n = 8). Using flow cytometry, we were also able to analyse TIM-3 protein expression in 10 patients and 6 healthy controls. RESULTS: A decreased TIM-3 mRNA (p < 0.05) and protein (p < 0.05) expression was observed in patients, and the level of TIM-3 mRNA correlated negatively with the CD4/CD8 T cell ratio in BALF cells of patients. Compared to a distinct subgroup of patients i.e. those with Löfgren's syndrome, BALF CD4(+ )T cells from non- Löfgren's patients expressed decreased mRNA levels of TIM-1 (p < 0.05). mRNA expression of IL-2 was increased in patients (p < 0.01) and non-Löfgren's patients expressed significantly higher levels of IFN-γ mRNA (p < 0.05) versus patients with Löfgren's syndrome. CONCLUSION: These findings are the first data on the expression of TIM-1 and TIM-3 molecules in sarcoidosis. The reduced TIM-3 expression in the lungs of patients may result in a defective T cell ability to control the Th1 immune response and could thus contribute to the pathogenesis of sarcoidosis. The down-regulated TIM-1 expression in non-Löfgren'spatients is in agreement with an exaggerated Th1 response in these patients. |
format | Text |
id | pubmed-2694180 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26941802009-06-09 Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4(+ )T cells in sarcoidosis Idali, Farah Wahlström, Jan Dahlberg, Benita Khademi, Mohsen Olsson, Tomas Eklund, Anders Grunewald, Johan Respir Res Research BACKGROUND: Activated T helper (Th)-1 pulmonary CD4(+ )cells and their mediators are essential for the inflammation and granulomatous process in sarcoidosis. Recently, T-cell immunoglobulin and mucin domain (TIM) molecules were suggested to be important regulators of immune function. In this study, we wanted to investigate whether TIM molecules could play a role in sarcoidosis. METHODS: We used real-time polymerase chain reaction to investigate the differential gene expression of TIM-1 and TIM-3 as well as a few Th1 and Th2 cytokines (IL-2, IFN-γ, IL-4, IL-5 and IL-13) in CD4(+ )T cells isolated from bronchoalveolar lavage fluid (BALF) of patients (n = 28) and healthy controls (n = 8). Using flow cytometry, we were also able to analyse TIM-3 protein expression in 10 patients and 6 healthy controls. RESULTS: A decreased TIM-3 mRNA (p < 0.05) and protein (p < 0.05) expression was observed in patients, and the level of TIM-3 mRNA correlated negatively with the CD4/CD8 T cell ratio in BALF cells of patients. Compared to a distinct subgroup of patients i.e. those with Löfgren's syndrome, BALF CD4(+ )T cells from non- Löfgren's patients expressed decreased mRNA levels of TIM-1 (p < 0.05). mRNA expression of IL-2 was increased in patients (p < 0.01) and non-Löfgren's patients expressed significantly higher levels of IFN-γ mRNA (p < 0.05) versus patients with Löfgren's syndrome. CONCLUSION: These findings are the first data on the expression of TIM-1 and TIM-3 molecules in sarcoidosis. The reduced TIM-3 expression in the lungs of patients may result in a defective T cell ability to control the Th1 immune response and could thus contribute to the pathogenesis of sarcoidosis. The down-regulated TIM-1 expression in non-Löfgren'spatients is in agreement with an exaggerated Th1 response in these patients. BioMed Central 2009 2009-05-29 /pmc/articles/PMC2694180/ /pubmed/19480659 http://dx.doi.org/10.1186/1465-9921-10-42 Text en Copyright © 2009 Idali et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Idali, Farah Wahlström, Jan Dahlberg, Benita Khademi, Mohsen Olsson, Tomas Eklund, Anders Grunewald, Johan Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4(+ )T cells in sarcoidosis |
title | Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4(+ )T cells in sarcoidosis |
title_full | Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4(+ )T cells in sarcoidosis |
title_fullStr | Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4(+ )T cells in sarcoidosis |
title_full_unstemmed | Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4(+ )T cells in sarcoidosis |
title_short | Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4(+ )T cells in sarcoidosis |
title_sort | altered expression of t cell immunoglobulin-mucin (tim) molecules in bronchoalveolar lavage cd4(+ )t cells in sarcoidosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2694180/ https://www.ncbi.nlm.nih.gov/pubmed/19480659 http://dx.doi.org/10.1186/1465-9921-10-42 |
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