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Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha

Actin plays fundamental roles in a wide array of plant functions, including cell division, cytoplasmic streaming, cell morphogenesis and organelle motility. Imaging the actin cytoskeleton in living cells is a powerful methodology for studying these important phenomena. Several useful probes for live...

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Autores principales: Era, Atsuko, Tominaga, Motoki, Ebine, Kazuo, Awai, Chie, Saito, Chieko, Ishizaki, Kimitsune, Yamato, Katsuyuki T., Kohchi, Takayuki, Nakano, Akihiko, Ueda, Takashi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2694730/
https://www.ncbi.nlm.nih.gov/pubmed/19369273
http://dx.doi.org/10.1093/pcp/pcp055
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author Era, Atsuko
Tominaga, Motoki
Ebine, Kazuo
Awai, Chie
Saito, Chieko
Ishizaki, Kimitsune
Yamato, Katsuyuki T.
Kohchi, Takayuki
Nakano, Akihiko
Ueda, Takashi
author_facet Era, Atsuko
Tominaga, Motoki
Ebine, Kazuo
Awai, Chie
Saito, Chieko
Ishizaki, Kimitsune
Yamato, Katsuyuki T.
Kohchi, Takayuki
Nakano, Akihiko
Ueda, Takashi
author_sort Era, Atsuko
collection PubMed
description Actin plays fundamental roles in a wide array of plant functions, including cell division, cytoplasmic streaming, cell morphogenesis and organelle motility. Imaging the actin cytoskeleton in living cells is a powerful methodology for studying these important phenomena. Several useful probes for live imaging of filamentous actin (F-actin) have been developed, but new versatile probes are still needed. Here, we report the application of a new probe called Lifeact for visualizing F-actin in plant cells. Lifeact is a short peptide comprising 17 amino acids that was derived from yeast Abp140p. We used a Lifeact–Venus fusion protein for staining F-actin in Arabidopsis thaliana and were able to observe dynamic rearrangements of the actin meshwork in root hair cells. We also used Lifeact–Venus to visualize the actin cytoskeleton in the liverwort Marchantia polymorpha; this revealed unique and dynamic F-actin motility in liverwort cells. Our results suggest that Lifeact could be a useful tool for studying the actin cytoskeleton in a wide range of plant lineages.
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spelling pubmed-26947302009-06-15 Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha Era, Atsuko Tominaga, Motoki Ebine, Kazuo Awai, Chie Saito, Chieko Ishizaki, Kimitsune Yamato, Katsuyuki T. Kohchi, Takayuki Nakano, Akihiko Ueda, Takashi Plant Cell Physiol Regular Papers Actin plays fundamental roles in a wide array of plant functions, including cell division, cytoplasmic streaming, cell morphogenesis and organelle motility. Imaging the actin cytoskeleton in living cells is a powerful methodology for studying these important phenomena. Several useful probes for live imaging of filamentous actin (F-actin) have been developed, but new versatile probes are still needed. Here, we report the application of a new probe called Lifeact for visualizing F-actin in plant cells. Lifeact is a short peptide comprising 17 amino acids that was derived from yeast Abp140p. We used a Lifeact–Venus fusion protein for staining F-actin in Arabidopsis thaliana and were able to observe dynamic rearrangements of the actin meshwork in root hair cells. We also used Lifeact–Venus to visualize the actin cytoskeleton in the liverwort Marchantia polymorpha; this revealed unique and dynamic F-actin motility in liverwort cells. Our results suggest that Lifeact could be a useful tool for studying the actin cytoskeleton in a wide range of plant lineages. Oxford University Press 2009-06 2009-04-20 /pmc/articles/PMC2694730/ /pubmed/19369273 http://dx.doi.org/10.1093/pcp/pcp055 Text en © The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org
spellingShingle Regular Papers
Era, Atsuko
Tominaga, Motoki
Ebine, Kazuo
Awai, Chie
Saito, Chieko
Ishizaki, Kimitsune
Yamato, Katsuyuki T.
Kohchi, Takayuki
Nakano, Akihiko
Ueda, Takashi
Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha
title Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha
title_full Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha
title_fullStr Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha
title_full_unstemmed Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha
title_short Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha
title_sort application of lifeact reveals f-actin dynamics in arabidopsis thaliana and the liverwort, marchantia polymorpha
topic Regular Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2694730/
https://www.ncbi.nlm.nih.gov/pubmed/19369273
http://dx.doi.org/10.1093/pcp/pcp055
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