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A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay
The aim of the study was to build a comprehensive picture of the appearance in the blood stream of Ag-specific plasma cells and memory B cells in the bovine model. For this purpose, we have developed a method allowing the detection and quantification of both cell types within individual calves immun...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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EDP Sciences
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695019/ https://www.ncbi.nlm.nih.gov/pubmed/18828984 http://dx.doi.org/10.1051/vetres:2008041 |
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author | Lefevre, Eric A. Carr, B. Veronica Prentice, Helen Charleston, Bryan |
author_facet | Lefevre, Eric A. Carr, B. Veronica Prentice, Helen Charleston, Bryan |
author_sort | Lefevre, Eric A. |
collection | PubMed |
description | The aim of the study was to build a comprehensive picture of the appearance in the blood stream of Ag-specific plasma cells and memory B cells in the bovine model. For this purpose, we have developed a method allowing the detection and quantification of both cell types within individual calves immunised with ovalbumin. During the primary response, we detected a burst of ovalbumin-specific plasma cells at days 6 and 7 post-immunisation, which was followed by the production of specific Ab, whereas a gradual increase of memory B cells was only detected from day 15. As expected, a boost immunisation performed 7 weeks later induced a quicker and stronger secondary response. Indeed, a burst of plasma cells was detected in the blood at days 3 and 4, which was followed by a strong increase in Ab titres. Furthermore, a burst of memory B cells, and not a gradual increase, was detected at days 5 and 6 post-boost immunisation. Importantly, we showed a strong correlation between the anti-ovalbumin-specific IgG titres detected 5 months after secondary immunisation and the plasma cell numbers detected in the blood at the peak response after secondary immunisation. The detection and quantification of plasma cells following an immunisation/vaccination strategy could constitute a very effective means for predicting the magnitude and longevity of an Ab response. |
format | Text |
id | pubmed-2695019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-26950192009-06-29 A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay Lefevre, Eric A. Carr, B. Veronica Prentice, Helen Charleston, Bryan Vet Res Original Article The aim of the study was to build a comprehensive picture of the appearance in the blood stream of Ag-specific plasma cells and memory B cells in the bovine model. For this purpose, we have developed a method allowing the detection and quantification of both cell types within individual calves immunised with ovalbumin. During the primary response, we detected a burst of ovalbumin-specific plasma cells at days 6 and 7 post-immunisation, which was followed by the production of specific Ab, whereas a gradual increase of memory B cells was only detected from day 15. As expected, a boost immunisation performed 7 weeks later induced a quicker and stronger secondary response. Indeed, a burst of plasma cells was detected in the blood at days 3 and 4, which was followed by a strong increase in Ab titres. Furthermore, a burst of memory B cells, and not a gradual increase, was detected at days 5 and 6 post-boost immunisation. Importantly, we showed a strong correlation between the anti-ovalbumin-specific IgG titres detected 5 months after secondary immunisation and the plasma cell numbers detected in the blood at the peak response after secondary immunisation. The detection and quantification of plasma cells following an immunisation/vaccination strategy could constitute a very effective means for predicting the magnitude and longevity of an Ab response. EDP Sciences 2009 2008-10-03 /pmc/articles/PMC2695019/ /pubmed/18828984 http://dx.doi.org/10.1051/vetres:2008041 Text en © INRA, EDP Sciences, 2008 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any noncommercial medium, provided the original work is properly cited. |
spellingShingle | Original Article Lefevre, Eric A. Carr, B. Veronica Prentice, Helen Charleston, Bryan A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay |
title | A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay |
title_full | A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay |
title_fullStr | A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay |
title_full_unstemmed | A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay |
title_short | A quantitative assessment of primary and secondary immune responses in cattle using a B cell ELISPOT assay |
title_sort | quantitative assessment of primary and secondary immune responses in cattle using a b cell elispot assay |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695019/ https://www.ncbi.nlm.nih.gov/pubmed/18828984 http://dx.doi.org/10.1051/vetres:2008041 |
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