Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability

The objective of this study was to elucidate the effects of hydroxylpropyl-β-cyclodextrin (HP-β-CD) on the in vitro stability of insulin. It was found that HP-β-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-β-CD cou...

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Detalles Bibliográficos
Autores principales: Zhang, Liefeng, Zhu, Wenjie, Song, Lingling, Wang, Yifan, Jiang, Hui, Xian, Suyun, Ren, Yong
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695265/
https://www.ncbi.nlm.nih.gov/pubmed/19564937
http://dx.doi.org/10.3390/ijms10052031
Descripción
Sumario:The objective of this study was to elucidate the effects of hydroxylpropyl-β-cyclodextrin (HP-β-CD) on the in vitro stability of insulin. It was found that HP-β-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-β-CD could also increase the stability of disulfide bonds which are important to the conformation of insulin. Through (1)H-NMR experiments it was found that the protective effect of HP-β-CD was due to complexation with insulin. The results suggest that the presence of HP-β-CD could improve the stability of insulin in different environments.

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