Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability

The objective of this study was to elucidate the effects of hydroxylpropyl-β-cyclodextrin (HP-β-CD) on the in vitro stability of insulin. It was found that HP-β-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-β-CD cou...

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Detalles Bibliográficos
Autores principales: Zhang, Liefeng, Zhu, Wenjie, Song, Lingling, Wang, Yifan, Jiang, Hui, Xian, Suyun, Ren, Yong
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695265/
https://www.ncbi.nlm.nih.gov/pubmed/19564937
http://dx.doi.org/10.3390/ijms10052031
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author Zhang, Liefeng
Zhu, Wenjie
Song, Lingling
Wang, Yifan
Jiang, Hui
Xian, Suyun
Ren, Yong
author_facet Zhang, Liefeng
Zhu, Wenjie
Song, Lingling
Wang, Yifan
Jiang, Hui
Xian, Suyun
Ren, Yong
author_sort Zhang, Liefeng
collection PubMed
description The objective of this study was to elucidate the effects of hydroxylpropyl-β-cyclodextrin (HP-β-CD) on the in vitro stability of insulin. It was found that HP-β-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-β-CD could also increase the stability of disulfide bonds which are important to the conformation of insulin. Through (1)H-NMR experiments it was found that the protective effect of HP-β-CD was due to complexation with insulin. The results suggest that the presence of HP-β-CD could improve the stability of insulin in different environments.
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spelling pubmed-26952652009-06-29 Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability Zhang, Liefeng Zhu, Wenjie Song, Lingling Wang, Yifan Jiang, Hui Xian, Suyun Ren, Yong Int J Mol Sci Article The objective of this study was to elucidate the effects of hydroxylpropyl-β-cyclodextrin (HP-β-CD) on the in vitro stability of insulin. It was found that HP-β-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-β-CD could also increase the stability of disulfide bonds which are important to the conformation of insulin. Through (1)H-NMR experiments it was found that the protective effect of HP-β-CD was due to complexation with insulin. The results suggest that the presence of HP-β-CD could improve the stability of insulin in different environments. Molecular Diversity Preservation International (MDPI) 2009-05-06 /pmc/articles/PMC2695265/ /pubmed/19564937 http://dx.doi.org/10.3390/ijms10052031 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhang, Liefeng
Zhu, Wenjie
Song, Lingling
Wang, Yifan
Jiang, Hui
Xian, Suyun
Ren, Yong
Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability
title Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability
title_full Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability
title_fullStr Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability
title_full_unstemmed Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability
title_short Effects of Hydroxylpropyl-β-Cyclodextrin on in Vitro Insulin Stability
title_sort effects of hydroxylpropyl-β-cyclodextrin on in vitro insulin stability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695265/
https://www.ncbi.nlm.nih.gov/pubmed/19564937
http://dx.doi.org/10.3390/ijms10052031
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AT wangyifan effectsofhydroxylpropylbcyclodextrinoninvitroinsulinstability
AT jianghui effectsofhydroxylpropylbcyclodextrinoninvitroinsulinstability
AT xiansuyun effectsofhydroxylpropylbcyclodextrinoninvitroinsulinstability
AT renyong effectsofhydroxylpropylbcyclodextrinoninvitroinsulinstability

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