Construction of an Artificial MicroRNA Expression Vector for Simultaneous Inhibition of Multiple Genes in Mammalian Cells

Recently, artificial microRNA (amiRNA) has become a promising RNA interference (RNAi) technology. Here, we describe a flexible and reliable method for constructing both single- and multi-amiRNA expression vectors. Two universal primers, together with two specific primers carrying the encoding sequen...

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Detalles Bibliográficos
Autores principales: Hu, Tao, Fu, Qiong, Chen, Ping, Ma, Li, Sin, Onsam, Guo, Deyin
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695274/
https://www.ncbi.nlm.nih.gov/pubmed/19564946
http://dx.doi.org/10.3390/ijms10052158

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