Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification

The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on in...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Linna, Gao, Hongwei, Li, Jiping, Dong, Ying, Liu, Ning, Wan, Jiayu, Liu, Wensen, Sun, Yucheng, Xu, Ming
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695285/
https://www.ncbi.nlm.nih.gov/pubmed/19564957
http://dx.doi.org/10.3390/ijms10052431
Descripción
Sumario:The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin.

Ejemplares similares