Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification

The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on in...

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Detalles Bibliográficos
Autores principales: Liu, Linna, Gao, Hongwei, Li, Jiping, Dong, Ying, Liu, Ning, Wan, Jiayu, Liu, Wensen, Sun, Yucheng, Xu, Ming
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695285/
https://www.ncbi.nlm.nih.gov/pubmed/19564957
http://dx.doi.org/10.3390/ijms10052431
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author Liu, Linna
Gao, Hongwei
Li, Jiping
Dong, Ying
Liu, Ning
Wan, Jiayu
Liu, Wensen
Sun, Yucheng
Xu, Ming
author_facet Liu, Linna
Gao, Hongwei
Li, Jiping
Dong, Ying
Liu, Ning
Wan, Jiayu
Liu, Wensen
Sun, Yucheng
Xu, Ming
author_sort Liu, Linna
collection PubMed
description The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin.
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spelling pubmed-26952852009-06-29 Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification Liu, Linna Gao, Hongwei Li, Jiping Dong, Ying Liu, Ning Wan, Jiayu Liu, Wensen Sun, Yucheng Xu, Ming Int J Mol Sci Article The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin. Molecular Diversity Preservation International (MDPI) 2009-05-22 /pmc/articles/PMC2695285/ /pubmed/19564957 http://dx.doi.org/10.3390/ijms10052431 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Liu, Linna
Gao, Hongwei
Li, Jiping
Dong, Ying
Liu, Ning
Wan, Jiayu
Liu, Wensen
Sun, Yucheng
Xu, Ming
Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_full Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_fullStr Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_full_unstemmed Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_short Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_sort analysis of intestinal injuries induced by ricin in vitro using spr technology and ms identification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695285/
https://www.ncbi.nlm.nih.gov/pubmed/19564957
http://dx.doi.org/10.3390/ijms10052431
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