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Mung Bean nuclease mapping of RNAs 3' end
A method is described that allows an accurate mapping of 3' ends of RNAs. In this method a labeled DNA probe, containing the presumed 3' end of the RNA under analysis is allowed to anneals to the RNA itself. Mung-bean nuclease is then used to digest single strands of both RNA and DNA. Elec...
Autores principales: | Bellavia, Daniele, Sisino, Giorgia, Papadopoulos, Giorgio L, Forte, Giusi I, Barbieri, Rainer |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695431/ https://www.ncbi.nlm.nih.gov/pubmed/19460154 http://dx.doi.org/10.1186/1742-4933-6-6 |
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