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The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate

BACKGROUND: Secondary metabolism in Serratia sp. ATCC 39006 (Serratia 39006) is controlled via a complex network of regulators, including a LuxIR-type (SmaIR) quorum sensing (QS) system. Here we investigate the molecular mechanism by which phosphate limitation controls biosynthesis of two antibiotic...

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Autores principales: Gristwood, Tamzin, Fineran, Peter C, Everson, Lee, Williamson, Neil R, Salmond, George P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695467/
https://www.ncbi.nlm.nih.gov/pubmed/19476633
http://dx.doi.org/10.1186/1471-2180-9-112
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author Gristwood, Tamzin
Fineran, Peter C
Everson, Lee
Williamson, Neil R
Salmond, George P
author_facet Gristwood, Tamzin
Fineran, Peter C
Everson, Lee
Williamson, Neil R
Salmond, George P
author_sort Gristwood, Tamzin
collection PubMed
description BACKGROUND: Secondary metabolism in Serratia sp. ATCC 39006 (Serratia 39006) is controlled via a complex network of regulators, including a LuxIR-type (SmaIR) quorum sensing (QS) system. Here we investigate the molecular mechanism by which phosphate limitation controls biosynthesis of two antibiotic secondary metabolites, prodigiosin and carbapenem, in Serratia 39006. RESULTS: We demonstrate that a mutation in the high affinity phosphate transporter pstSCAB-phoU, believed to mimic low phosphate conditions, causes upregulation of secondary metabolism and QS in Serratia 39006, via the PhoBR two-component system. Phosphate limitation also activated secondary metabolism and QS in Serratia 39006. In addition, a pstS mutation resulted in upregulation of rap. Rap, a putative SlyA/MarR-family transcriptional regulator, shares similarity with the global regulator RovA (regulator of virulence) from Yersina spp. and is an activator of secondary metabolism in Serratia 39006. We demonstrate that expression of rap, pigA-O (encoding the prodigiosin biosynthetic operon) and smaI are controlled via PhoBR in Serratia 39006. CONCLUSION: Phosphate limitation regulates secondary metabolism in Serratia 39006 via multiple inter-linked pathways, incorporating transcriptional control mediated by three important global regulators, PhoB, SmaR and Rap.
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spelling pubmed-26954672009-06-12 The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate Gristwood, Tamzin Fineran, Peter C Everson, Lee Williamson, Neil R Salmond, George P BMC Microbiol Research article BACKGROUND: Secondary metabolism in Serratia sp. ATCC 39006 (Serratia 39006) is controlled via a complex network of regulators, including a LuxIR-type (SmaIR) quorum sensing (QS) system. Here we investigate the molecular mechanism by which phosphate limitation controls biosynthesis of two antibiotic secondary metabolites, prodigiosin and carbapenem, in Serratia 39006. RESULTS: We demonstrate that a mutation in the high affinity phosphate transporter pstSCAB-phoU, believed to mimic low phosphate conditions, causes upregulation of secondary metabolism and QS in Serratia 39006, via the PhoBR two-component system. Phosphate limitation also activated secondary metabolism and QS in Serratia 39006. In addition, a pstS mutation resulted in upregulation of rap. Rap, a putative SlyA/MarR-family transcriptional regulator, shares similarity with the global regulator RovA (regulator of virulence) from Yersina spp. and is an activator of secondary metabolism in Serratia 39006. We demonstrate that expression of rap, pigA-O (encoding the prodigiosin biosynthetic operon) and smaI are controlled via PhoBR in Serratia 39006. CONCLUSION: Phosphate limitation regulates secondary metabolism in Serratia 39006 via multiple inter-linked pathways, incorporating transcriptional control mediated by three important global regulators, PhoB, SmaR and Rap. BioMed Central 2009-05-28 /pmc/articles/PMC2695467/ /pubmed/19476633 http://dx.doi.org/10.1186/1471-2180-9-112 Text en Copyright ©2009 Gristwood et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Gristwood, Tamzin
Fineran, Peter C
Everson, Lee
Williamson, Neil R
Salmond, George P
The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate
title The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate
title_full The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate
title_fullStr The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate
title_full_unstemmed The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate
title_short The PhoBR two-component system regulates antibiotic biosynthesis in Serratia in response to phosphate
title_sort phobr two-component system regulates antibiotic biosynthesis in serratia in response to phosphate
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695467/
https://www.ncbi.nlm.nih.gov/pubmed/19476633
http://dx.doi.org/10.1186/1471-2180-9-112
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