A first generation bovine BAC-based physical map

A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26...

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Autores principales: Schibler, Laurent, Roig, Anne, Mahé, Marie-Françoise, Save, Jean-Claude, Gautier, Mathieu, Taourit, Sead, Boichard, Didier, Eggen, André, Cribiu, Edmond P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2697175/
https://www.ncbi.nlm.nih.gov/pubmed/14713413
http://dx.doi.org/10.1186/1297-9686-36-1-105
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author Schibler, Laurent
Roig, Anne
Mahé, Marie-Françoise
Save, Jean-Claude
Gautier, Mathieu
Taourit, Sead
Boichard, Didier
Eggen, André
Cribiu, Edmond P
author_facet Schibler, Laurent
Roig, Anne
Mahé, Marie-Françoise
Save, Jean-Claude
Gautier, Mathieu
Taourit, Sead
Boichard, Didier
Eggen, André
Cribiu, Edmond P
author_sort Schibler, Laurent
collection PubMed
description A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.
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spelling pubmed-26971752009-06-16 A first generation bovine BAC-based physical map Schibler, Laurent Roig, Anne Mahé, Marie-Françoise Save, Jean-Claude Gautier, Mathieu Taourit, Sead Boichard, Didier Eggen, André Cribiu, Edmond P Genet Sel Evol Research A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest. BioMed Central 2004-01-15 /pmc/articles/PMC2697175/ /pubmed/14713413 http://dx.doi.org/10.1186/1297-9686-36-1-105 Text en Copyright © 2004 INRA, EDP Sciences
spellingShingle Research
Schibler, Laurent
Roig, Anne
Mahé, Marie-Françoise
Save, Jean-Claude
Gautier, Mathieu
Taourit, Sead
Boichard, Didier
Eggen, André
Cribiu, Edmond P
A first generation bovine BAC-based physical map
title A first generation bovine BAC-based physical map
title_full A first generation bovine BAC-based physical map
title_fullStr A first generation bovine BAC-based physical map
title_full_unstemmed A first generation bovine BAC-based physical map
title_short A first generation bovine BAC-based physical map
title_sort first generation bovine bac-based physical map
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2697175/
https://www.ncbi.nlm.nih.gov/pubmed/14713413
http://dx.doi.org/10.1186/1297-9686-36-1-105
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