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Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney

Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from ren...

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Detalles Bibliográficos
Autores principales: Walker, Louise A., MacCallum, Donna M., Bertram, Gwyneth, Gow, Neil A.R., Odds, Frank C., Brown, Alistair J.P.
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698078/
https://www.ncbi.nlm.nih.gov/pubmed/19032986
http://dx.doi.org/10.1016/j.fgb.2008.10.012
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author Walker, Louise A.
MacCallum, Donna M.
Bertram, Gwyneth
Gow, Neil A.R.
Odds, Frank C.
Brown, Alistair J.P.
author_facet Walker, Louise A.
MacCallum, Donna M.
Bertram, Gwyneth
Gow, Neil A.R.
Odds, Frank C.
Brown, Alistair J.P.
author_sort Walker, Louise A.
collection PubMed
description Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.
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spelling pubmed-26980782009-06-23 Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney Walker, Louise A. MacCallum, Donna M. Bertram, Gwyneth Gow, Neil A.R. Odds, Frank C. Brown, Alistair J.P. Fungal Genet Biol Article Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested. Academic Press 2009-02 /pmc/articles/PMC2698078/ /pubmed/19032986 http://dx.doi.org/10.1016/j.fgb.2008.10.012 Text en © 2009 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Walker, Louise A.
MacCallum, Donna M.
Bertram, Gwyneth
Gow, Neil A.R.
Odds, Frank C.
Brown, Alistair J.P.
Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney
title Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney
title_full Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney
title_fullStr Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney
title_full_unstemmed Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney
title_short Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney
title_sort genome-wide analysis of candida albicans gene expression patterns during infection of the mammalian kidney
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698078/
https://www.ncbi.nlm.nih.gov/pubmed/19032986
http://dx.doi.org/10.1016/j.fgb.2008.10.012
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