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Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney
Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from ren...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Academic Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698078/ https://www.ncbi.nlm.nih.gov/pubmed/19032986 http://dx.doi.org/10.1016/j.fgb.2008.10.012 |
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author | Walker, Louise A. MacCallum, Donna M. Bertram, Gwyneth Gow, Neil A.R. Odds, Frank C. Brown, Alistair J.P. |
author_facet | Walker, Louise A. MacCallum, Donna M. Bertram, Gwyneth Gow, Neil A.R. Odds, Frank C. Brown, Alistair J.P. |
author_sort | Walker, Louise A. |
collection | PubMed |
description | Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested. |
format | Text |
id | pubmed-2698078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-26980782009-06-23 Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney Walker, Louise A. MacCallum, Donna M. Bertram, Gwyneth Gow, Neil A.R. Odds, Frank C. Brown, Alistair J.P. Fungal Genet Biol Article Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested. Academic Press 2009-02 /pmc/articles/PMC2698078/ /pubmed/19032986 http://dx.doi.org/10.1016/j.fgb.2008.10.012 Text en © 2009 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Walker, Louise A. MacCallum, Donna M. Bertram, Gwyneth Gow, Neil A.R. Odds, Frank C. Brown, Alistair J.P. Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney |
title | Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney |
title_full | Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney |
title_fullStr | Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney |
title_full_unstemmed | Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney |
title_short | Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney |
title_sort | genome-wide analysis of candida albicans gene expression patterns during infection of the mammalian kidney |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698078/ https://www.ncbi.nlm.nih.gov/pubmed/19032986 http://dx.doi.org/10.1016/j.fgb.2008.10.012 |
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