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Quantification of Food Intake in Drosophila

Measurement of food intake in the fruit fly Drosophila melanogaster is often necessary for studies of behaviour, nutrition and drug administration. There is no reliable and agreed method for measuring food intake of flies in undisturbed, steady state, and normal culture conditions. We report such a...

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Detalles Bibliográficos
Autores principales: Wong, Richard, Piper, Matthew D. W., Wertheim, Bregje, Partridge, Linda
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698149/
https://www.ncbi.nlm.nih.gov/pubmed/19557170
http://dx.doi.org/10.1371/journal.pone.0006063
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author Wong, Richard
Piper, Matthew D. W.
Wertheim, Bregje
Partridge, Linda
author_facet Wong, Richard
Piper, Matthew D. W.
Wertheim, Bregje
Partridge, Linda
author_sort Wong, Richard
collection PubMed
description Measurement of food intake in the fruit fly Drosophila melanogaster is often necessary for studies of behaviour, nutrition and drug administration. There is no reliable and agreed method for measuring food intake of flies in undisturbed, steady state, and normal culture conditions. We report such a method, based on measurement of feeding frequency by proboscis-extension, validated by short-term measurements of food dye intake. We used the method to demonstrate that (a) female flies feed more frequently than males, (b) flies feed more often when housed in larger groups and (c) fly feeding varies at different times of the day. We also show that alterations in food intake are not induced by dietary restriction or by a null mutation of the fly insulin receptor substrate chico. In contrast, mutation of takeout increases food intake by increasing feeding frequency while mutation of ovo(D) increases food intake by increasing the volume of food consumed per proboscis-extension. This approach provides a practical and reliable method for quantification of food intake in Drosophila under normal, undisturbed culture conditions.
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spelling pubmed-26981492009-06-26 Quantification of Food Intake in Drosophila Wong, Richard Piper, Matthew D. W. Wertheim, Bregje Partridge, Linda PLoS One Research Article Measurement of food intake in the fruit fly Drosophila melanogaster is often necessary for studies of behaviour, nutrition and drug administration. There is no reliable and agreed method for measuring food intake of flies in undisturbed, steady state, and normal culture conditions. We report such a method, based on measurement of feeding frequency by proboscis-extension, validated by short-term measurements of food dye intake. We used the method to demonstrate that (a) female flies feed more frequently than males, (b) flies feed more often when housed in larger groups and (c) fly feeding varies at different times of the day. We also show that alterations in food intake are not induced by dietary restriction or by a null mutation of the fly insulin receptor substrate chico. In contrast, mutation of takeout increases food intake by increasing feeding frequency while mutation of ovo(D) increases food intake by increasing the volume of food consumed per proboscis-extension. This approach provides a practical and reliable method for quantification of food intake in Drosophila under normal, undisturbed culture conditions. Public Library of Science 2009-06-26 /pmc/articles/PMC2698149/ /pubmed/19557170 http://dx.doi.org/10.1371/journal.pone.0006063 Text en Wong et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wong, Richard
Piper, Matthew D. W.
Wertheim, Bregje
Partridge, Linda
Quantification of Food Intake in Drosophila
title Quantification of Food Intake in Drosophila
title_full Quantification of Food Intake in Drosophila
title_fullStr Quantification of Food Intake in Drosophila
title_full_unstemmed Quantification of Food Intake in Drosophila
title_short Quantification of Food Intake in Drosophila
title_sort quantification of food intake in drosophila
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2698149/
https://www.ncbi.nlm.nih.gov/pubmed/19557170
http://dx.doi.org/10.1371/journal.pone.0006063
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