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Characterisation of the expression and function of the GM-CSF receptor α-chain in mice
The granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic cytokine able to regulate a variety of cell functions including differentiation of macrophages and granulocytes, dendritic cell development and the maintenance of homeostasis. It binds specifically to its receptor, whic...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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WILEY-VCH Verlag
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699419/ https://www.ncbi.nlm.nih.gov/pubmed/17694571 http://dx.doi.org/10.1002/eji.200636892 |
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author | Rosas, Marcela Gordon, Siamon Taylor, Philip R |
author_facet | Rosas, Marcela Gordon, Siamon Taylor, Philip R |
author_sort | Rosas, Marcela |
collection | PubMed |
description | The granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic cytokine able to regulate a variety of cell functions including differentiation of macrophages and granulocytes, dendritic cell development and the maintenance of homeostasis. It binds specifically to its receptor, which is composed of a cytokine-specific α-chain (GM-CSF receptor α-chain, GMRα) and a β-chain shared with the receptors for interleukin-3 and interleukin-5. In this report, we present a comprehensive study of GMRα in the mouse. We have found that the mouse GMRα is polymorphic and alternatively spliced. In the absence of specific antibodies, we generated a novel chimeric protein containing the Fc fragment of human IgG1 coupled to mouse GM-CSF, which was able to specifically bind to GMRα and induce proliferation of GMRα-transduced Ba/F3 cells. We used this reagent to perform the first detailed FACS study of the surface expression of mouse GMRα by leucocytes. Highest expression was found on monocytes and granulocytes, and variable expression on tissue macrophages. The GM-CSF receptor in mice is specifically expressed by myeloid cells and is useful for the detection of novel uncharacterised myeloid populations. The ability to detect GM-CSF receptor expression in experimental studies should greatly facilitate the analysis of its role in immune pathologies. |
format | Text |
id | pubmed-2699419 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | WILEY-VCH Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-26994192009-06-25 Characterisation of the expression and function of the GM-CSF receptor α-chain in mice Rosas, Marcela Gordon, Siamon Taylor, Philip R Eur J Immunol Innate immunity The granulocyte-macrophage colony-stimulating factor (GM-CSF) is a hematopoietic cytokine able to regulate a variety of cell functions including differentiation of macrophages and granulocytes, dendritic cell development and the maintenance of homeostasis. It binds specifically to its receptor, which is composed of a cytokine-specific α-chain (GM-CSF receptor α-chain, GMRα) and a β-chain shared with the receptors for interleukin-3 and interleukin-5. In this report, we present a comprehensive study of GMRα in the mouse. We have found that the mouse GMRα is polymorphic and alternatively spliced. In the absence of specific antibodies, we generated a novel chimeric protein containing the Fc fragment of human IgG1 coupled to mouse GM-CSF, which was able to specifically bind to GMRα and induce proliferation of GMRα-transduced Ba/F3 cells. We used this reagent to perform the first detailed FACS study of the surface expression of mouse GMRα by leucocytes. Highest expression was found on monocytes and granulocytes, and variable expression on tissue macrophages. The GM-CSF receptor in mice is specifically expressed by myeloid cells and is useful for the detection of novel uncharacterised myeloid populations. The ability to detect GM-CSF receptor expression in experimental studies should greatly facilitate the analysis of its role in immune pathologies. WILEY-VCH Verlag 2007-09 /pmc/articles/PMC2699419/ /pubmed/17694571 http://dx.doi.org/10.1002/eji.200636892 Text en Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Innate immunity Rosas, Marcela Gordon, Siamon Taylor, Philip R Characterisation of the expression and function of the GM-CSF receptor α-chain in mice |
title | Characterisation of the expression and function of the GM-CSF receptor α-chain in mice |
title_full | Characterisation of the expression and function of the GM-CSF receptor α-chain in mice |
title_fullStr | Characterisation of the expression and function of the GM-CSF receptor α-chain in mice |
title_full_unstemmed | Characterisation of the expression and function of the GM-CSF receptor α-chain in mice |
title_short | Characterisation of the expression and function of the GM-CSF receptor α-chain in mice |
title_sort | characterisation of the expression and function of the gm-csf receptor α-chain in mice |
topic | Innate immunity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699419/ https://www.ncbi.nlm.nih.gov/pubmed/17694571 http://dx.doi.org/10.1002/eji.200636892 |
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