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CD8 exerts differential effects on the deployment of cytotoxic T lymphocyte effector functions
Cytotoxic T lymphocytes (CTL) are equipped with a range of effector functions that contribute both to the control of intracellular pathogens and dysregulated cellular proliferation and to the development of certain immunopathologies such as autoimmune disease. Qualitative analyses of various CTL res...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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WILEY-VCH Verlag
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699424/ https://www.ncbi.nlm.nih.gov/pubmed/17393387 http://dx.doi.org/10.1002/eji.200636718 |
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author | Laugel, Bruno Price, David A Milicic, Anita Sewell, Andrew K |
author_facet | Laugel, Bruno Price, David A Milicic, Anita Sewell, Andrew K |
author_sort | Laugel, Bruno |
collection | PubMed |
description | Cytotoxic T lymphocytes (CTL) are equipped with a range of effector functions that contribute both to the control of intracellular pathogens and dysregulated cellular proliferation and to the development of certain immunopathologies such as autoimmune disease. Qualitative analyses of various CTL responses have revealed substantial heterogeneity in the diversity of functions that are mobilized in response to antigen. Here, we studied the influence of the CD8 co-receptor, which is known to enhance antigen recognition by CTL, on the secretion of eight different cytokines and chemokines by human CTL clones using flow cytometric bead array. Our results show that abrogation of MHC class I/CD8 interactions exerts a differential influence on the distinct individual effector functions that are elicited in response to agonist ligands. The magnitude of this co-receptor blockade inhibitory effect was clearly related to the hierarchy of cytokine secretion in terms of activation threshold because those functions requiring the highest amounts of antigen were most affected. Thus, modulation of CD8 activity can effectively tune not only the sensitivity but also the qualitative profile of CTL responses. |
format | Text |
id | pubmed-2699424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | WILEY-VCH Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-26994242009-06-25 CD8 exerts differential effects on the deployment of cytotoxic T lymphocyte effector functions Laugel, Bruno Price, David A Milicic, Anita Sewell, Andrew K Eur J Immunol Cellular immune response Cytotoxic T lymphocytes (CTL) are equipped with a range of effector functions that contribute both to the control of intracellular pathogens and dysregulated cellular proliferation and to the development of certain immunopathologies such as autoimmune disease. Qualitative analyses of various CTL responses have revealed substantial heterogeneity in the diversity of functions that are mobilized in response to antigen. Here, we studied the influence of the CD8 co-receptor, which is known to enhance antigen recognition by CTL, on the secretion of eight different cytokines and chemokines by human CTL clones using flow cytometric bead array. Our results show that abrogation of MHC class I/CD8 interactions exerts a differential influence on the distinct individual effector functions that are elicited in response to agonist ligands. The magnitude of this co-receptor blockade inhibitory effect was clearly related to the hierarchy of cytokine secretion in terms of activation threshold because those functions requiring the highest amounts of antigen were most affected. Thus, modulation of CD8 activity can effectively tune not only the sensitivity but also the qualitative profile of CTL responses. WILEY-VCH Verlag 2007-04 /pmc/articles/PMC2699424/ /pubmed/17393387 http://dx.doi.org/10.1002/eji.200636718 Text en Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Cellular immune response Laugel, Bruno Price, David A Milicic, Anita Sewell, Andrew K CD8 exerts differential effects on the deployment of cytotoxic T lymphocyte effector functions |
title | CD8 exerts differential effects on the deployment of cytotoxic T lymphocyte effector functions |
title_full | CD8 exerts differential effects on the deployment of cytotoxic T lymphocyte effector functions |
title_fullStr | CD8 exerts differential effects on the deployment of cytotoxic T lymphocyte effector functions |
title_full_unstemmed | CD8 exerts differential effects on the deployment of cytotoxic T lymphocyte effector functions |
title_short | CD8 exerts differential effects on the deployment of cytotoxic T lymphocyte effector functions |
title_sort | cd8 exerts differential effects on the deployment of cytotoxic t lymphocyte effector functions |
topic | Cellular immune response |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699424/ https://www.ncbi.nlm.nih.gov/pubmed/17393387 http://dx.doi.org/10.1002/eji.200636718 |
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