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Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s)
The “RNA revolution” that started at the end of the 20th century with the discovery of post-transcriptional gene silencing and its mechanism via RNA interference (RNAi) placed tiny 21-24 nucleotide long noncoding RNAs (ncRNAs) in the forefront of biology as one of the most important regulatory eleme...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699438/ https://www.ncbi.nlm.nih.gov/pubmed/19551152 http://dx.doi.org/10.1155/2009/915061 |
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author | Devor, Eric J. Huang, Lingyan Abdukarimov, Abdusattor Abdurakhmonov, Ibrokhim Y. |
author_facet | Devor, Eric J. Huang, Lingyan Abdukarimov, Abdusattor Abdurakhmonov, Ibrokhim Y. |
author_sort | Devor, Eric J. |
collection | PubMed |
description | The “RNA revolution” that started at the end of the 20th century with the discovery of post-transcriptional gene silencing and its mechanism via RNA interference (RNAi) placed tiny 21-24 nucleotide long noncoding RNAs (ncRNAs) in the forefront of biology as one of the most important regulatory elements in a host of physiologic processes. The discovery of new classes of ncRNAs including endogenous small interfering RNAs, microRNAs, and PIWI-interacting RNAs is a hallmark in the understanding of RNA-dependent gene regulation. New generation high-throughput sequencing technologies further accelerated the studies of this “tiny world” and provided their global characterization and validation in many biological systems with sequenced genomes. Nevertheless, for the many “yet-unsequenced” plant genomes, the discovery of small RNA world requires in vitro cloning from purified cellular RNAs. Thus, reproducible methods for in vitro small RNA cloning are of paramount importance and will remain so into the foreseeable future. In this paper, we present a description of existing small RNA cloning methods as well as next-generation sequencing methods that have accelerated this research along with a description of the application of one in vitro cloning method in an initial small RNA survey in the “still unsequenced” allotetraploid cotton genome. |
format | Text |
id | pubmed-2699438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-26994382009-06-23 Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s) Devor, Eric J. Huang, Lingyan Abdukarimov, Abdusattor Abdurakhmonov, Ibrokhim Y. Int J Plant Genomics Review Article The “RNA revolution” that started at the end of the 20th century with the discovery of post-transcriptional gene silencing and its mechanism via RNA interference (RNAi) placed tiny 21-24 nucleotide long noncoding RNAs (ncRNAs) in the forefront of biology as one of the most important regulatory elements in a host of physiologic processes. The discovery of new classes of ncRNAs including endogenous small interfering RNAs, microRNAs, and PIWI-interacting RNAs is a hallmark in the understanding of RNA-dependent gene regulation. New generation high-throughput sequencing technologies further accelerated the studies of this “tiny world” and provided their global characterization and validation in many biological systems with sequenced genomes. Nevertheless, for the many “yet-unsequenced” plant genomes, the discovery of small RNA world requires in vitro cloning from purified cellular RNAs. Thus, reproducible methods for in vitro small RNA cloning are of paramount importance and will remain so into the foreseeable future. In this paper, we present a description of existing small RNA cloning methods as well as next-generation sequencing methods that have accelerated this research along with a description of the application of one in vitro cloning method in an initial small RNA survey in the “still unsequenced” allotetraploid cotton genome. Hindawi Publishing Corporation 2009 2009-06-15 /pmc/articles/PMC2699438/ /pubmed/19551152 http://dx.doi.org/10.1155/2009/915061 Text en Copyright © 2009 Eric J. Devor et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Devor, Eric J. Huang, Lingyan Abdukarimov, Abdusattor Abdurakhmonov, Ibrokhim Y. Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s) |
title | Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s) |
title_full | Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s) |
title_fullStr | Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s) |
title_full_unstemmed | Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s) |
title_short | Methodologies for In Vitro Cloning of Small RNAs and Application for Plant Genome(s) |
title_sort | methodologies for in vitro cloning of small rnas and application for plant genome(s) |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699438/ https://www.ncbi.nlm.nih.gov/pubmed/19551152 http://dx.doi.org/10.1155/2009/915061 |
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