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Misregulation of rhodopsin phosphorylation and dephosphorylation found in P23H rat retinal degeneration

To examine rhodopsin (Rho) functions in P23H rat, kinetics of Rho regeneration and dephosphorylation were investigated by spectrophotometric analysis and immunofluorescence labeling method using specific antibodies toward phosphorylated 334Ser or 338Ser site. Rho dephosphorylation at both sites was...

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Autores principales: Saito, Yoshiyuki, Ohguro, Hiroshi, Ohguro, Ikuyo, Sato, Noriyuki, Ishikawa, Futoshi, Yamazaki, Hitoshi, Metoki, Tomomi, Ito, Tadashi, Nakazawa, Mitsuru
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699806/
https://www.ncbi.nlm.nih.gov/pubmed/19668436
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author Saito, Yoshiyuki
Ohguro, Hiroshi
Ohguro, Ikuyo
Sato, Noriyuki
Ishikawa, Futoshi
Yamazaki, Hitoshi
Metoki, Tomomi
Ito, Tadashi
Nakazawa, Mitsuru
author_facet Saito, Yoshiyuki
Ohguro, Hiroshi
Ohguro, Ikuyo
Sato, Noriyuki
Ishikawa, Futoshi
Yamazaki, Hitoshi
Metoki, Tomomi
Ito, Tadashi
Nakazawa, Mitsuru
author_sort Saito, Yoshiyuki
collection PubMed
description To examine rhodopsin (Rho) functions in P23H rat, kinetics of Rho regeneration and dephosphorylation were investigated by spectrophotometric analysis and immunofluorescence labeling method using specific antibodies toward phosphorylated 334Ser or 338Ser site. Rho dephosphorylation at both sites was extremely delayed in P23H retina as compared to normal ones. Kinetics of Rho regeneration was not altered between normal and P23H rats under dark adaptation. Next, to study the effects of several Ca(2+)channel blockers on this model, retinal function and morphology were evaluated. Among them, nilvadipine showed a significant protective effect against P23H retinal degeneration. Neurotrophic factor, fibroblast growth factor-2 and Arc, known to suppress the apoptosis in the central nervous system, were significantly upregulated upon administration of nilvadipine. The present study indicates that misregulation of Rho phosphorylation may be involved as an important step in retinal degeneration of P23H and administration of nilvadipine may be a potential therapeutic agent for the retinal degenerations.
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spelling pubmed-26998062009-08-10 Misregulation of rhodopsin phosphorylation and dephosphorylation found in P23H rat retinal degeneration Saito, Yoshiyuki Ohguro, Hiroshi Ohguro, Ikuyo Sato, Noriyuki Ishikawa, Futoshi Yamazaki, Hitoshi Metoki, Tomomi Ito, Tadashi Nakazawa, Mitsuru Clin Ophthalmol Original Research To examine rhodopsin (Rho) functions in P23H rat, kinetics of Rho regeneration and dephosphorylation were investigated by spectrophotometric analysis and immunofluorescence labeling method using specific antibodies toward phosphorylated 334Ser or 338Ser site. Rho dephosphorylation at both sites was extremely delayed in P23H retina as compared to normal ones. Kinetics of Rho regeneration was not altered between normal and P23H rats under dark adaptation. Next, to study the effects of several Ca(2+)channel blockers on this model, retinal function and morphology were evaluated. Among them, nilvadipine showed a significant protective effect against P23H retinal degeneration. Neurotrophic factor, fibroblast growth factor-2 and Arc, known to suppress the apoptosis in the central nervous system, were significantly upregulated upon administration of nilvadipine. The present study indicates that misregulation of Rho phosphorylation may be involved as an important step in retinal degeneration of P23H and administration of nilvadipine may be a potential therapeutic agent for the retinal degenerations. Dove Medical Press 2008-12 2008-12 /pmc/articles/PMC2699806/ /pubmed/19668436 Text en © 2008 Saito et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Saito, Yoshiyuki
Ohguro, Hiroshi
Ohguro, Ikuyo
Sato, Noriyuki
Ishikawa, Futoshi
Yamazaki, Hitoshi
Metoki, Tomomi
Ito, Tadashi
Nakazawa, Mitsuru
Misregulation of rhodopsin phosphorylation and dephosphorylation found in P23H rat retinal degeneration
title Misregulation of rhodopsin phosphorylation and dephosphorylation found in P23H rat retinal degeneration
title_full Misregulation of rhodopsin phosphorylation and dephosphorylation found in P23H rat retinal degeneration
title_fullStr Misregulation of rhodopsin phosphorylation and dephosphorylation found in P23H rat retinal degeneration
title_full_unstemmed Misregulation of rhodopsin phosphorylation and dephosphorylation found in P23H rat retinal degeneration
title_short Misregulation of rhodopsin phosphorylation and dephosphorylation found in P23H rat retinal degeneration
title_sort misregulation of rhodopsin phosphorylation and dephosphorylation found in p23h rat retinal degeneration
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699806/
https://www.ncbi.nlm.nih.gov/pubmed/19668436
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