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Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways
Global deciphering of signal transduction pathways represents a new challenge of the post-genomic era. However, for the majority of these signaling pathways the role(s), the function(s) and the interaction(s) of the signaling intermediates remain to be characterized in an integrated fashion. The glo...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Bentham Science Publishers Ltd.
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699825/ https://www.ncbi.nlm.nih.gov/pubmed/19794881 http://dx.doi.org/10.2174/138920209787847041 |
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author | Taouji, Saïd Dahan, Sophie Bossé, Roger Chevet, Eric |
author_facet | Taouji, Saïd Dahan, Sophie Bossé, Roger Chevet, Eric |
author_sort | Taouji, Saïd |
collection | PubMed |
description | Global deciphering of signal transduction pathways represents a new challenge of the post-genomic era. However, for the majority of these signaling pathways the role(s), the function(s) and the interaction(s) of the signaling intermediates remain to be characterized in an integrated fashion. The global molecular study of cell signaling pathways and networks consequently requires sensitive, robust technologies which may allow in addition multi-parallel and highthroughput applications. The Alphascreen™ technology, relying on a bead-based homogenous approach, constitutes a valuable tool to detect and quantify a wide range of signaling events such as enzymatic activities or biomolecular interactions. In this article, we exhaustively review the literature and report the broad spectrum of Alphascreen™-based applications in the study of signal transduction pathways. |
format | Text |
id | pubmed-2699825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Bentham Science Publishers Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-26998252009-10-01 Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways Taouji, Saïd Dahan, Sophie Bossé, Roger Chevet, Eric Curr Genomics Article Global deciphering of signal transduction pathways represents a new challenge of the post-genomic era. However, for the majority of these signaling pathways the role(s), the function(s) and the interaction(s) of the signaling intermediates remain to be characterized in an integrated fashion. The global molecular study of cell signaling pathways and networks consequently requires sensitive, robust technologies which may allow in addition multi-parallel and highthroughput applications. The Alphascreen™ technology, relying on a bead-based homogenous approach, constitutes a valuable tool to detect and quantify a wide range of signaling events such as enzymatic activities or biomolecular interactions. In this article, we exhaustively review the literature and report the broad spectrum of Alphascreen™-based applications in the study of signal transduction pathways. Bentham Science Publishers Ltd. 2009-04 /pmc/articles/PMC2699825/ /pubmed/19794881 http://dx.doi.org/10.2174/138920209787847041 Text en ©2009 Bentham Science Publishers Ltd. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Taouji, Saïd Dahan, Sophie Bossé, Roger Chevet, Eric Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways |
title | Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways |
title_full | Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways |
title_fullStr | Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways |
title_full_unstemmed | Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways |
title_short | Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways |
title_sort | current screens based on the alphascreen™ technology for deciphering cell signalling pathways |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699825/ https://www.ncbi.nlm.nih.gov/pubmed/19794881 http://dx.doi.org/10.2174/138920209787847041 |
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