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Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways

Global deciphering of signal transduction pathways represents a new challenge of the post-genomic era. However, for the majority of these signaling pathways the role(s), the function(s) and the interaction(s) of the signaling intermediates remain to be characterized in an integrated fashion. The glo...

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Detalles Bibliográficos
Autores principales: Taouji, Saïd, Dahan, Sophie, Bossé, Roger, Chevet, Eric
Formato: Texto
Lenguaje:English
Publicado: Bentham Science Publishers Ltd. 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699825/
https://www.ncbi.nlm.nih.gov/pubmed/19794881
http://dx.doi.org/10.2174/138920209787847041
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author Taouji, Saïd
Dahan, Sophie
Bossé, Roger
Chevet, Eric
author_facet Taouji, Saïd
Dahan, Sophie
Bossé, Roger
Chevet, Eric
author_sort Taouji, Saïd
collection PubMed
description Global deciphering of signal transduction pathways represents a new challenge of the post-genomic era. However, for the majority of these signaling pathways the role(s), the function(s) and the interaction(s) of the signaling intermediates remain to be characterized in an integrated fashion. The global molecular study of cell signaling pathways and networks consequently requires sensitive, robust technologies which may allow in addition multi-parallel and highthroughput applications. The Alphascreen™ technology, relying on a bead-based homogenous approach, constitutes a valuable tool to detect and quantify a wide range of signaling events such as enzymatic activities or biomolecular interactions. In this article, we exhaustively review the literature and report the broad spectrum of Alphascreen™-based applications in the study of signal transduction pathways.
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spelling pubmed-26998252009-10-01 Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways Taouji, Saïd Dahan, Sophie Bossé, Roger Chevet, Eric Curr Genomics Article Global deciphering of signal transduction pathways represents a new challenge of the post-genomic era. However, for the majority of these signaling pathways the role(s), the function(s) and the interaction(s) of the signaling intermediates remain to be characterized in an integrated fashion. The global molecular study of cell signaling pathways and networks consequently requires sensitive, robust technologies which may allow in addition multi-parallel and highthroughput applications. The Alphascreen™ technology, relying on a bead-based homogenous approach, constitutes a valuable tool to detect and quantify a wide range of signaling events such as enzymatic activities or biomolecular interactions. In this article, we exhaustively review the literature and report the broad spectrum of Alphascreen™-based applications in the study of signal transduction pathways. Bentham Science Publishers Ltd. 2009-04 /pmc/articles/PMC2699825/ /pubmed/19794881 http://dx.doi.org/10.2174/138920209787847041 Text en ©2009 Bentham Science Publishers Ltd. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Taouji, Saïd
Dahan, Sophie
Bossé, Roger
Chevet, Eric
Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways
title Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways
title_full Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways
title_fullStr Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways
title_full_unstemmed Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways
title_short Current Screens Based on the AlphaScreen™ Technology for Deciphering Cell Signalling Pathways
title_sort current screens based on the alphascreen™ technology for deciphering cell signalling pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2699825/
https://www.ncbi.nlm.nih.gov/pubmed/19794881
http://dx.doi.org/10.2174/138920209787847041
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