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Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid

BACKGROUND: Streptococcus agalactiae (group B Streptococcus) is a bacterial pathogen that causes severe intrauterine infections leading to fetal morbidity and mortality. The pathogenesis of GBS infection in this environment is poorly understood, in part because we lack a detailed understanding of th...

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Autores principales: Sitkiewicz, Izabela, Green, Nicole M., Guo, Nina, Bongiovanni, Ann Marie, Witkin, Steven S., Musser, James M.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700258/
https://www.ncbi.nlm.nih.gov/pubmed/19568429
http://dx.doi.org/10.1371/journal.pone.0006114
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author Sitkiewicz, Izabela
Green, Nicole M.
Guo, Nina
Bongiovanni, Ann Marie
Witkin, Steven S.
Musser, James M.
author_facet Sitkiewicz, Izabela
Green, Nicole M.
Guo, Nina
Bongiovanni, Ann Marie
Witkin, Steven S.
Musser, James M.
author_sort Sitkiewicz, Izabela
collection PubMed
description BACKGROUND: Streptococcus agalactiae (group B Streptococcus) is a bacterial pathogen that causes severe intrauterine infections leading to fetal morbidity and mortality. The pathogenesis of GBS infection in this environment is poorly understood, in part because we lack a detailed understanding of the adaptation of this pathogen to growth in amniotic fluid. To address this knowledge deficit, we characterized the transcriptome of GBS grown in human amniotic fluid (AF) and compared it with the transcriptome in rich laboratory medium. METHODS: GBS was grown in Todd Hewitt-yeast extract medium and human AF. Bacteria were collected at mid-logarithmic, late-logarithmic and stationary growth phase. We performed global expression microarray analysis using a custom-made Affymetrix GeneChip. The normalized hybridization values derived from three biological replicates at each growth point were obtained. AF/THY transcript ratios representing greater than a 2-fold change and P-value exceeding 0.05 were considered to be statistically significant. PRINCIPAL FINDINGS: We have discovered that GBS significantly remodels its transcriptome in response to exposure to human amniotic fluid. GBS grew rapidly in human AF and did not exhibit a global stress response. The majority of changes in GBS transcripts in AF compared to THY medium were related to genes mediating metabolism of amino acids, carbohydrates, and nucleotides. The majority of the observed changes in transcripts affects genes involved in basic bacterial metabolism and is connected to AF composition and nutritional requirements of the bacterium. Importantly, the response to growth in human AF included significant changes in transcripts of multiple virulence genes such as adhesins, capsule, and hemolysin and IL-8 proteinase what might have consequences for the outcome of host-pathogen interactions. CONCLUSIONS/SIGNIFICANCE: Our work provides extensive new information about how the transcriptome of GBS responds to growth in AF, and thus new leads for pathogenesis research.
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spelling pubmed-27002582009-07-01 Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid Sitkiewicz, Izabela Green, Nicole M. Guo, Nina Bongiovanni, Ann Marie Witkin, Steven S. Musser, James M. PLoS One Research Article BACKGROUND: Streptococcus agalactiae (group B Streptococcus) is a bacterial pathogen that causes severe intrauterine infections leading to fetal morbidity and mortality. The pathogenesis of GBS infection in this environment is poorly understood, in part because we lack a detailed understanding of the adaptation of this pathogen to growth in amniotic fluid. To address this knowledge deficit, we characterized the transcriptome of GBS grown in human amniotic fluid (AF) and compared it with the transcriptome in rich laboratory medium. METHODS: GBS was grown in Todd Hewitt-yeast extract medium and human AF. Bacteria were collected at mid-logarithmic, late-logarithmic and stationary growth phase. We performed global expression microarray analysis using a custom-made Affymetrix GeneChip. The normalized hybridization values derived from three biological replicates at each growth point were obtained. AF/THY transcript ratios representing greater than a 2-fold change and P-value exceeding 0.05 were considered to be statistically significant. PRINCIPAL FINDINGS: We have discovered that GBS significantly remodels its transcriptome in response to exposure to human amniotic fluid. GBS grew rapidly in human AF and did not exhibit a global stress response. The majority of changes in GBS transcripts in AF compared to THY medium were related to genes mediating metabolism of amino acids, carbohydrates, and nucleotides. The majority of the observed changes in transcripts affects genes involved in basic bacterial metabolism and is connected to AF composition and nutritional requirements of the bacterium. Importantly, the response to growth in human AF included significant changes in transcripts of multiple virulence genes such as adhesins, capsule, and hemolysin and IL-8 proteinase what might have consequences for the outcome of host-pathogen interactions. CONCLUSIONS/SIGNIFICANCE: Our work provides extensive new information about how the transcriptome of GBS responds to growth in AF, and thus new leads for pathogenesis research. Public Library of Science 2009-07-01 /pmc/articles/PMC2700258/ /pubmed/19568429 http://dx.doi.org/10.1371/journal.pone.0006114 Text en Sitkiewicz et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sitkiewicz, Izabela
Green, Nicole M.
Guo, Nina
Bongiovanni, Ann Marie
Witkin, Steven S.
Musser, James M.
Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid
title Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid
title_full Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid
title_fullStr Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid
title_full_unstemmed Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid
title_short Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid
title_sort transcriptome adaptation of group b streptococcus to growth in human amniotic fluid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700258/
https://www.ncbi.nlm.nih.gov/pubmed/19568429
http://dx.doi.org/10.1371/journal.pone.0006114
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