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Dynamic Imaging of CD8(+) T Cells and Dendritic Cells during Infection with Toxoplasma gondii

To better understand the initiation of CD8(+) T cell responses during infection, the primary response to the intracellular parasite Toxoplasma gondii was characterized using 2-photon microscopy combined with an experimental system that allowed visualization of dendritic cells (DCs) and parasite spec...

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Autores principales: John, Beena, Harris, Tajie H., Tait, Elia D., Wilson, Emma H., Gregg, Beth, Ng, Lai Guan, Mrass, Paulus, Roos, David S., Dzierszinski, Florence, Weninger, Wolfgang, Hunter, Christopher A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700268/
https://www.ncbi.nlm.nih.gov/pubmed/19578440
http://dx.doi.org/10.1371/journal.ppat.1000505
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author John, Beena
Harris, Tajie H.
Tait, Elia D.
Wilson, Emma H.
Gregg, Beth
Ng, Lai Guan
Mrass, Paulus
Roos, David S.
Dzierszinski, Florence
Weninger, Wolfgang
Hunter, Christopher A.
author_facet John, Beena
Harris, Tajie H.
Tait, Elia D.
Wilson, Emma H.
Gregg, Beth
Ng, Lai Guan
Mrass, Paulus
Roos, David S.
Dzierszinski, Florence
Weninger, Wolfgang
Hunter, Christopher A.
author_sort John, Beena
collection PubMed
description To better understand the initiation of CD8(+) T cell responses during infection, the primary response to the intracellular parasite Toxoplasma gondii was characterized using 2-photon microscopy combined with an experimental system that allowed visualization of dendritic cells (DCs) and parasite specific CD8(+) T cells. Infection with T. gondii induced localization of both these populations to the sub-capsular/interfollicular region of the draining lymph node and DCs were required for the expansion of the T cells. Consistent with current models, in the presence of cognate antigen, the average velocity of CD8(+) T cells decreased. Unexpectedly, infection also resulted in modulation of the behavior of non-parasite specific T cells. This TCR-independent process correlated with the re-modeling of the lymph node micro-architecture and changes in expression of CCL21 and CCL3. Infection also resulted in sustained interactions between the DCs and CD8(+) T cells that were visualized only in the presence of cognate antigen and were limited to an early phase in the response. Infected DCs were rare within the lymph node during this time frame; however, DCs presenting the cognate antigen were detected. Together, these data provide novel insights into the earliest interaction between DCs and CD8(+) T cells and suggest that cross presentation by bystander DCs rather than infected DCs is an important route of antigen presentation during toxoplasmosis.
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spelling pubmed-27002682009-07-03 Dynamic Imaging of CD8(+) T Cells and Dendritic Cells during Infection with Toxoplasma gondii John, Beena Harris, Tajie H. Tait, Elia D. Wilson, Emma H. Gregg, Beth Ng, Lai Guan Mrass, Paulus Roos, David S. Dzierszinski, Florence Weninger, Wolfgang Hunter, Christopher A. PLoS Pathog Research Article To better understand the initiation of CD8(+) T cell responses during infection, the primary response to the intracellular parasite Toxoplasma gondii was characterized using 2-photon microscopy combined with an experimental system that allowed visualization of dendritic cells (DCs) and parasite specific CD8(+) T cells. Infection with T. gondii induced localization of both these populations to the sub-capsular/interfollicular region of the draining lymph node and DCs were required for the expansion of the T cells. Consistent with current models, in the presence of cognate antigen, the average velocity of CD8(+) T cells decreased. Unexpectedly, infection also resulted in modulation of the behavior of non-parasite specific T cells. This TCR-independent process correlated with the re-modeling of the lymph node micro-architecture and changes in expression of CCL21 and CCL3. Infection also resulted in sustained interactions between the DCs and CD8(+) T cells that were visualized only in the presence of cognate antigen and were limited to an early phase in the response. Infected DCs were rare within the lymph node during this time frame; however, DCs presenting the cognate antigen were detected. Together, these data provide novel insights into the earliest interaction between DCs and CD8(+) T cells and suggest that cross presentation by bystander DCs rather than infected DCs is an important route of antigen presentation during toxoplasmosis. Public Library of Science 2009-07-03 /pmc/articles/PMC2700268/ /pubmed/19578440 http://dx.doi.org/10.1371/journal.ppat.1000505 Text en John et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
John, Beena
Harris, Tajie H.
Tait, Elia D.
Wilson, Emma H.
Gregg, Beth
Ng, Lai Guan
Mrass, Paulus
Roos, David S.
Dzierszinski, Florence
Weninger, Wolfgang
Hunter, Christopher A.
Dynamic Imaging of CD8(+) T Cells and Dendritic Cells during Infection with Toxoplasma gondii
title Dynamic Imaging of CD8(+) T Cells and Dendritic Cells during Infection with Toxoplasma gondii
title_full Dynamic Imaging of CD8(+) T Cells and Dendritic Cells during Infection with Toxoplasma gondii
title_fullStr Dynamic Imaging of CD8(+) T Cells and Dendritic Cells during Infection with Toxoplasma gondii
title_full_unstemmed Dynamic Imaging of CD8(+) T Cells and Dendritic Cells during Infection with Toxoplasma gondii
title_short Dynamic Imaging of CD8(+) T Cells and Dendritic Cells during Infection with Toxoplasma gondii
title_sort dynamic imaging of cd8(+) t cells and dendritic cells during infection with toxoplasma gondii
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700268/
https://www.ncbi.nlm.nih.gov/pubmed/19578440
http://dx.doi.org/10.1371/journal.ppat.1000505
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