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Characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options

BACKGROUND: Coagulase-negative staphylococci (CoNS) are a major cause of nosocomial blood stream infection, especially in critically ill and haematology patients. CoNS are usually multidrug-resistant and glycopeptide antibiotics have been to date considered the drugs of choice for treatment. The aim...

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Autores principales: Natoli, Silvia, Fontana, Carla, Favaro, Marco, Bergamini, Alberto, Testore, Gian Piero, Minelli, Silvia, Bossa, Maria Cristina, Casapulla, Mauro, Broglio, Giorgia, Beltrame, Angela, Cudillo, Laura, Cerretti, Raffaella, Leonardis, Francesca
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701436/
https://www.ncbi.nlm.nih.gov/pubmed/19497104
http://dx.doi.org/10.1186/1471-2334-9-83
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author Natoli, Silvia
Fontana, Carla
Favaro, Marco
Bergamini, Alberto
Testore, Gian Piero
Minelli, Silvia
Bossa, Maria Cristina
Casapulla, Mauro
Broglio, Giorgia
Beltrame, Angela
Cudillo, Laura
Cerretti, Raffaella
Leonardis, Francesca
author_facet Natoli, Silvia
Fontana, Carla
Favaro, Marco
Bergamini, Alberto
Testore, Gian Piero
Minelli, Silvia
Bossa, Maria Cristina
Casapulla, Mauro
Broglio, Giorgia
Beltrame, Angela
Cudillo, Laura
Cerretti, Raffaella
Leonardis, Francesca
author_sort Natoli, Silvia
collection PubMed
description BACKGROUND: Coagulase-negative staphylococci (CoNS) are a major cause of nosocomial blood stream infection, especially in critically ill and haematology patients. CoNS are usually multidrug-resistant and glycopeptide antibiotics have been to date considered the drugs of choice for treatment. The aim of this study was to characterize CoNS with reduced susceptibility to glycopeptides causing blood stream infection (BSI) in critically ill and haematology patients at the University Hospital Tor Vergata, Rome, Italy, in 2007. METHODS: Hospital microbiology records for transplant haematology and ICU were reviewed to identify CoNS with elevated MICs for glycopeptides, and isolates were matched to clinical records to determine whether the isolates caused a BSI. The isolates were tested for susceptibility to new drugs daptomicin and tigecycline and the genetic relationship was assessed using f-AFLP. RESULTS: Of a total of 17,418 blood cultures, 1,609 were positive for CoNS and of these, 87 (5.4%) displayed reduced susceptibility to glycopeptides. Clinical review revealed that in 13 cases (7 in haematology and 6 in ICU), CoNS with reduced susceptibility to glycopeptides were responsible for a BSI. Staphylococcus epidermidis was the causative organism in 11 instances and Staphylococcus haemolyticus in 2. The incidence of oxacillin resistance was high (77%), although all isolates remained susceptible to linezolid, daptomycin and tigecycline. Fingerprinting of CoNS identified one clonal relationship between two isolates. CONCLUSION: Multi-resistant CoNS with reduced susceptibility to glycopeptides, although still relatively infrequent in our hospital, are emerging pathogens of clinical concern. Surveillance by antibiotyping with attention to multi-resistant profile, and warning to clinicians, is necessary.
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spelling pubmed-27014362009-06-25 Characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options Natoli, Silvia Fontana, Carla Favaro, Marco Bergamini, Alberto Testore, Gian Piero Minelli, Silvia Bossa, Maria Cristina Casapulla, Mauro Broglio, Giorgia Beltrame, Angela Cudillo, Laura Cerretti, Raffaella Leonardis, Francesca BMC Infect Dis Research Article BACKGROUND: Coagulase-negative staphylococci (CoNS) are a major cause of nosocomial blood stream infection, especially in critically ill and haematology patients. CoNS are usually multidrug-resistant and glycopeptide antibiotics have been to date considered the drugs of choice for treatment. The aim of this study was to characterize CoNS with reduced susceptibility to glycopeptides causing blood stream infection (BSI) in critically ill and haematology patients at the University Hospital Tor Vergata, Rome, Italy, in 2007. METHODS: Hospital microbiology records for transplant haematology and ICU were reviewed to identify CoNS with elevated MICs for glycopeptides, and isolates were matched to clinical records to determine whether the isolates caused a BSI. The isolates were tested for susceptibility to new drugs daptomicin and tigecycline and the genetic relationship was assessed using f-AFLP. RESULTS: Of a total of 17,418 blood cultures, 1,609 were positive for CoNS and of these, 87 (5.4%) displayed reduced susceptibility to glycopeptides. Clinical review revealed that in 13 cases (7 in haematology and 6 in ICU), CoNS with reduced susceptibility to glycopeptides were responsible for a BSI. Staphylococcus epidermidis was the causative organism in 11 instances and Staphylococcus haemolyticus in 2. The incidence of oxacillin resistance was high (77%), although all isolates remained susceptible to linezolid, daptomycin and tigecycline. Fingerprinting of CoNS identified one clonal relationship between two isolates. CONCLUSION: Multi-resistant CoNS with reduced susceptibility to glycopeptides, although still relatively infrequent in our hospital, are emerging pathogens of clinical concern. Surveillance by antibiotyping with attention to multi-resistant profile, and warning to clinicians, is necessary. BioMed Central 2009-06-04 /pmc/articles/PMC2701436/ /pubmed/19497104 http://dx.doi.org/10.1186/1471-2334-9-83 Text en Copyright ©2009 Natoli et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Natoli, Silvia
Fontana, Carla
Favaro, Marco
Bergamini, Alberto
Testore, Gian Piero
Minelli, Silvia
Bossa, Maria Cristina
Casapulla, Mauro
Broglio, Giorgia
Beltrame, Angela
Cudillo, Laura
Cerretti, Raffaella
Leonardis, Francesca
Characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options
title Characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options
title_full Characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options
title_fullStr Characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options
title_full_unstemmed Characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options
title_short Characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options
title_sort characterization of coagulase-negative staphylococcal isolates from blood with reduced susceptibility to glycopeptides and therapeutic options
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701436/
https://www.ncbi.nlm.nih.gov/pubmed/19497104
http://dx.doi.org/10.1186/1471-2334-9-83
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