An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy

Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam...

Descripción completa

Detalles Bibliográficos
Autores principales: van Oven, Carel, Krawczyk, Przemek M., Stap, Jan, Melo, Arline M., Piazzetta, Maria H. O., Gobbi, Angelo L., van Veen, Henk A., Verhoeven, Jan, Aten, Jacob A.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701496/
https://www.ncbi.nlm.nih.gov/pubmed/19495740
http://dx.doi.org/10.1007/s00249-009-0472-7
Descripción
Sumario:Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.

Ejemplares similares