An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy

Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam...

Descripción completa

Detalles Bibliográficos
Autores principales: van Oven, Carel, Krawczyk, Przemek M., Stap, Jan, Melo, Arline M., Piazzetta, Maria H. O., Gobbi, Angelo L., van Veen, Henk A., Verhoeven, Jan, Aten, Jacob A.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701496/
https://www.ncbi.nlm.nih.gov/pubmed/19495740
http://dx.doi.org/10.1007/s00249-009-0472-7
_version_ 1782168704930283520
author van Oven, Carel
Krawczyk, Przemek M.
Stap, Jan
Melo, Arline M.
Piazzetta, Maria H. O.
Gobbi, Angelo L.
van Veen, Henk A.
Verhoeven, Jan
Aten, Jacob A.
author_facet van Oven, Carel
Krawczyk, Przemek M.
Stap, Jan
Melo, Arline M.
Piazzetta, Maria H. O.
Gobbi, Angelo L.
van Veen, Henk A.
Verhoeven, Jan
Aten, Jacob A.
author_sort van Oven, Carel
collection PubMed
description Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.
format Text
id pubmed-2701496
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-27014962009-06-29 An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy van Oven, Carel Krawczyk, Przemek M. Stap, Jan Melo, Arline M. Piazzetta, Maria H. O. Gobbi, Angelo L. van Veen, Henk A. Verhoeven, Jan Aten, Jacob A. Eur Biophys J Original Paper Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage. Springer-Verlag 2009-06-03 2009-07 /pmc/articles/PMC2701496/ /pubmed/19495740 http://dx.doi.org/10.1007/s00249-009-0472-7 Text en © The Author(s) 2009
spellingShingle Original Paper
van Oven, Carel
Krawczyk, Przemek M.
Stap, Jan
Melo, Arline M.
Piazzetta, Maria H. O.
Gobbi, Angelo L.
van Veen, Henk A.
Verhoeven, Jan
Aten, Jacob A.
An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy
title An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy
title_full An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy
title_fullStr An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy
title_full_unstemmed An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy
title_short An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy
title_sort ultrasoft x-ray multi-microbeam irradiation system for studies of dna damage responses by fixed- and live-cell fluorescence microscopy
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701496/
https://www.ncbi.nlm.nih.gov/pubmed/19495740
http://dx.doi.org/10.1007/s00249-009-0472-7
work_keys_str_mv AT vanovencarel anultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT krawczykprzemekm anultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT stapjan anultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT meloarlinem anultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT piazzettamariaho anultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT gobbiangelol anultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT vanveenhenka anultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT verhoevenjan anultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT atenjacoba anultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT vanovencarel ultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT krawczykprzemekm ultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT stapjan ultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT meloarlinem ultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT piazzettamariaho ultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT gobbiangelol ultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT vanveenhenka ultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT verhoevenjan ultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy
AT atenjacoba ultrasoftxraymultimicrobeamirradiationsystemforstudiesofdnadamageresponsesbyfixedandlivecellfluorescencemicroscopy

Ejemplares similares