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Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol

BACKGROUND: Mycobacterium tuberculosis DNA topoisomerase I is an attractive target for discovery of novel TB drugs that act by enhancing the accumulation of the topoisomerase-DNA cleavage product. It shares a common transesterification domain with other type IA DNA topoisomerases. There is, however,...

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Detalles Bibliográficos
Autores principales:	Annamalai, Thirunavukkarasu, Dani, Neil, Cheng, Bokun, Tse-Dinh, Yuk-Ching
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2702276/ https://www.ncbi.nlm.nih.gov/pubmed/19519900 http://dx.doi.org/10.1186/1471-2091-10-18

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