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Probing the endosperm gene expression landscape in Brassica napus

BACKGROUND: In species with exalbuminous seeds, the endosperm is eventually consumed and its space occupied by the embryo during seed development. However, the main constituent of the early developing seed is the liquid endosperm, and a significant portion of the carbon resources for the ensuing sta...

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Autores principales: Huang, Yi, Chen, Liang, Wang, Liping, Vijayan, Kannan, Phan, Sieu, Liu, Ziying, Wan, Lianglu, Ross, Andrew, Xiang, Daoquan, Datla, Raju, Pan, Youlian, Zou, Jitao
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2702316/
https://www.ncbi.nlm.nih.gov/pubmed/19490642
http://dx.doi.org/10.1186/1471-2164-10-256
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author Huang, Yi
Chen, Liang
Wang, Liping
Vijayan, Kannan
Phan, Sieu
Liu, Ziying
Wan, Lianglu
Ross, Andrew
Xiang, Daoquan
Datla, Raju
Pan, Youlian
Zou, Jitao
author_facet Huang, Yi
Chen, Liang
Wang, Liping
Vijayan, Kannan
Phan, Sieu
Liu, Ziying
Wan, Lianglu
Ross, Andrew
Xiang, Daoquan
Datla, Raju
Pan, Youlian
Zou, Jitao
author_sort Huang, Yi
collection PubMed
description BACKGROUND: In species with exalbuminous seeds, the endosperm is eventually consumed and its space occupied by the embryo during seed development. However, the main constituent of the early developing seed is the liquid endosperm, and a significant portion of the carbon resources for the ensuing stages of seed development arrive at the embryo through the endosperm. In contrast to the extensive study of species with persistent endosperm, little is known about the global gene expression pattern in the endosperm of exalbuminous seed species such as crucifer oilseeds. RESULTS: We took a multiparallel approach that combines ESTs, protein profiling and microarray analyses to look into the gene expression landscape in the endosperm of the oilseed crop Brassica napus. An EST collection of over 30,000 entries allowed us to detect close to 10,000 unisequences expressed in the endosperm. A protein profile analysis of more than 800 proteins corroborated several signature pathways uncovered by abundant ESTs. Using microarray analyses, we identified genes that are differentially or highly expressed across all developmental stages. These complementary analyses provided insight on several prominent metabolic pathways in the endosperm. We also discovered that a transcription factor LEAFY COTYLEDON (LEC1) was highly expressed in the endosperm and that the regulatory cascade downstream of LEC1 operates in the endosperm. CONCLUSION: The endosperm EST collection and the microarray dataset provide a basic genomic resource for dissecting metabolic and developmental events important for oilseed improvement. Our findings on the featured metabolic processes and the LEC1 regulatory cascade offer new angles for investigation on the integration of endosperm gene expression with embryo development and storage product deposition in seed development.
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spelling pubmed-27023162009-06-27 Probing the endosperm gene expression landscape in Brassica napus Huang, Yi Chen, Liang Wang, Liping Vijayan, Kannan Phan, Sieu Liu, Ziying Wan, Lianglu Ross, Andrew Xiang, Daoquan Datla, Raju Pan, Youlian Zou, Jitao BMC Genomics Research Article BACKGROUND: In species with exalbuminous seeds, the endosperm is eventually consumed and its space occupied by the embryo during seed development. However, the main constituent of the early developing seed is the liquid endosperm, and a significant portion of the carbon resources for the ensuing stages of seed development arrive at the embryo through the endosperm. In contrast to the extensive study of species with persistent endosperm, little is known about the global gene expression pattern in the endosperm of exalbuminous seed species such as crucifer oilseeds. RESULTS: We took a multiparallel approach that combines ESTs, protein profiling and microarray analyses to look into the gene expression landscape in the endosperm of the oilseed crop Brassica napus. An EST collection of over 30,000 entries allowed us to detect close to 10,000 unisequences expressed in the endosperm. A protein profile analysis of more than 800 proteins corroborated several signature pathways uncovered by abundant ESTs. Using microarray analyses, we identified genes that are differentially or highly expressed across all developmental stages. These complementary analyses provided insight on several prominent metabolic pathways in the endosperm. We also discovered that a transcription factor LEAFY COTYLEDON (LEC1) was highly expressed in the endosperm and that the regulatory cascade downstream of LEC1 operates in the endosperm. CONCLUSION: The endosperm EST collection and the microarray dataset provide a basic genomic resource for dissecting metabolic and developmental events important for oilseed improvement. Our findings on the featured metabolic processes and the LEC1 regulatory cascade offer new angles for investigation on the integration of endosperm gene expression with embryo development and storage product deposition in seed development. BioMed Central 2009-06-02 /pmc/articles/PMC2702316/ /pubmed/19490642 http://dx.doi.org/10.1186/1471-2164-10-256 Text en Copyright © 2009 Huang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huang, Yi
Chen, Liang
Wang, Liping
Vijayan, Kannan
Phan, Sieu
Liu, Ziying
Wan, Lianglu
Ross, Andrew
Xiang, Daoquan
Datla, Raju
Pan, Youlian
Zou, Jitao
Probing the endosperm gene expression landscape in Brassica napus
title Probing the endosperm gene expression landscape in Brassica napus
title_full Probing the endosperm gene expression landscape in Brassica napus
title_fullStr Probing the endosperm gene expression landscape in Brassica napus
title_full_unstemmed Probing the endosperm gene expression landscape in Brassica napus
title_short Probing the endosperm gene expression landscape in Brassica napus
title_sort probing the endosperm gene expression landscape in brassica napus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2702316/
https://www.ncbi.nlm.nih.gov/pubmed/19490642
http://dx.doi.org/10.1186/1471-2164-10-256
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