Modification of the IFCC reference measurement procedure for determination of HbA(1c) by HPLC-ESI-MS

The reference measurement procedure for determination of HbA(1c) (glycated haemoglobin) using HPLC(high performance liquid chromatography)-ESI(electrospray ionisation)-MS(mass spectrometry) has been modified. Main modifications were a change in the buffer composition of the HPLC, a change in the gra...

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Detalles Bibliográficos
Autores principales: Kaiser, Patricia, Akerboom, Theodorus, Dux, László, Reinauer, Hans
Formato: Texto
Lenguaje:English
Publicado: German Medical Science GMS Publishing House 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703220/
https://www.ncbi.nlm.nih.gov/pubmed/19675697
Descripción
Sumario:The reference measurement procedure for determination of HbA(1c) (glycated haemoglobin) using HPLC(high performance liquid chromatography)-ESI(electrospray ionisation)-MS(mass spectrometry) has been modified. Main modifications were a change in the buffer composition of the HPLC, a change in the gradient elution profile and the introduction of a post-column splitting system. The long-term stability of the HPLC-ESI-MS system proved to be of high importance to get reproducible results. Using the modified HPLC conditions stable retention times, high peak symmetry and a good column batch-to-batch reproducibility of the analytical results were obtained. The mean deviation from the target values was less than 0.7% with an imprecision of less than 2%.

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