Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates

Canine plasminogen is made up of seven domains. In each domain there are several cysteines that are linked by disulfide bonds. Reduction of a limited number of the cystines destabilizes the protein such that it precipitates. The bond or bonds that are broken provide about 14 kcal of stabilization en...

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Detalles Bibliográficos
Autor principal: Kornblatt, Jack A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703797/
https://www.ncbi.nlm.nih.gov/pubmed/19593387
http://dx.doi.org/10.1371/journal.pone.0006196
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author Kornblatt, Jack A.
author_facet Kornblatt, Jack A.
author_sort Kornblatt, Jack A.
collection PubMed
description Canine plasminogen is made up of seven domains. In each domain there are several cysteines that are linked by disulfide bonds. Reduction of a limited number of the cystines destabilizes the protein such that it precipitates. The bond or bonds that are broken provide about 14 kcal of stabilization energy. Circular dichroism and dynamic light scattering indicate that there is probably an intermediate that is formed prior to precipitation and that the intermediate is somewhat larger than the compact form of plasminogen.
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spelling pubmed-27037972009-07-10 Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates Kornblatt, Jack A. PLoS One Research Article Canine plasminogen is made up of seven domains. In each domain there are several cysteines that are linked by disulfide bonds. Reduction of a limited number of the cystines destabilizes the protein such that it precipitates. The bond or bonds that are broken provide about 14 kcal of stabilization energy. Circular dichroism and dynamic light scattering indicate that there is probably an intermediate that is formed prior to precipitation and that the intermediate is somewhat larger than the compact form of plasminogen. Public Library of Science 2009-07-10 /pmc/articles/PMC2703797/ /pubmed/19593387 http://dx.doi.org/10.1371/journal.pone.0006196 Text en Kornblatt. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kornblatt, Jack A.
Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates
title Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates
title_full Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates
title_fullStr Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates
title_full_unstemmed Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates
title_short Reduction of Canine Plasminogen Leads to an Expanded Molecule which Precipitates
title_sort reduction of canine plasminogen leads to an expanded molecule which precipitates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703797/
https://www.ncbi.nlm.nih.gov/pubmed/19593387
http://dx.doi.org/10.1371/journal.pone.0006196
work_keys_str_mv AT kornblattjacka reductionofcanineplasminogenleadstoanexpandedmoleculewhichprecipitates

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