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Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii
Many details of structure, function and substrate specificity of eukaryotic proteasomal systems have been elucidated. This information far-exceeds that available for the archaeal and bacterial counterparts. While structural and functional studies have provided some insight into the workings of proka...
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Formato: | Texto |
Lenguaje: | English |
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Blackwell Publishing Ltd
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2704935/ https://www.ncbi.nlm.nih.gov/pubmed/19284414 http://dx.doi.org/10.1111/j.1574-6968.2009.01547.x |
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author | Kirkland, P Aaron Maupin-Furlow, Julie A |
author_facet | Kirkland, P Aaron Maupin-Furlow, Julie A |
author_sort | Kirkland, P Aaron |
collection | PubMed |
description | Many details of structure, function and substrate specificity of eukaryotic proteasomal systems have been elucidated. This information far-exceeds that available for the archaeal and bacterial counterparts. While structural and functional studies have provided some insight into the workings of prokaryotic proteasomes, the question of substrate targeting and global cellular influence remain largely unaddressed. In this communication, we report an over 720-fold increase in the half-life of the DNA-sliding clamp protein proliferating cell nuclear antigen after knockout of the panA gene, encoding a proteasome-activating nucleotidase A, on the chromosome of the halophilic archaeon Haloferax volcanii. This discovery marks the first identification of a protein stabilized by an archaeal proteasome mutation and provides a starting point for investigations into substrate recognition mechanisms. The findings also begin to address the functional role of proteasomal systems within the scope of the archaeal cell. |
format | Text |
id | pubmed-2704935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-27049352009-07-13 Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii Kirkland, P Aaron Maupin-Furlow, Julie A FEMS Microbiol Lett Research Letters Many details of structure, function and substrate specificity of eukaryotic proteasomal systems have been elucidated. This information far-exceeds that available for the archaeal and bacterial counterparts. While structural and functional studies have provided some insight into the workings of prokaryotic proteasomes, the question of substrate targeting and global cellular influence remain largely unaddressed. In this communication, we report an over 720-fold increase in the half-life of the DNA-sliding clamp protein proliferating cell nuclear antigen after knockout of the panA gene, encoding a proteasome-activating nucleotidase A, on the chromosome of the halophilic archaeon Haloferax volcanii. This discovery marks the first identification of a protein stabilized by an archaeal proteasome mutation and provides a starting point for investigations into substrate recognition mechanisms. The findings also begin to address the functional role of proteasomal systems within the scope of the archaeal cell. Blackwell Publishing Ltd 2009-05 2009-03-05 /pmc/articles/PMC2704935/ /pubmed/19284414 http://dx.doi.org/10.1111/j.1574-6968.2009.01547.x Text en © 2009 The Authors. Journal compilation © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Research Letters Kirkland, P Aaron Maupin-Furlow, Julie A Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii |
title | Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii |
title_full | Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii |
title_fullStr | Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii |
title_full_unstemmed | Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii |
title_short | Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii |
title_sort | stabilization of an archaeal dna-sliding clamp protein, pcna, by proteasome-activating nucleotidase gene knockout in haloferax volcanii |
topic | Research Letters |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2704935/ https://www.ncbi.nlm.nih.gov/pubmed/19284414 http://dx.doi.org/10.1111/j.1574-6968.2009.01547.x |
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