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Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses

BACKGROUND: The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders. In this study, the molecular mechanisms involved in the response to acute and strenuous exercise were inves...

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Autores principales: Cappelli, Katia, Felicetti, Michela, Capomaccio, Stefano, Pieramati, Camillo, Silvestrelli, Maurizio, Verini-Supplizi, Andrea
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705340/
https://www.ncbi.nlm.nih.gov/pubmed/19552796
http://dx.doi.org/10.1186/1472-6793-9-12
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author Cappelli, Katia
Felicetti, Michela
Capomaccio, Stefano
Pieramati, Camillo
Silvestrelli, Maurizio
Verini-Supplizi, Andrea
author_facet Cappelli, Katia
Felicetti, Michela
Capomaccio, Stefano
Pieramati, Camillo
Silvestrelli, Maurizio
Verini-Supplizi, Andrea
author_sort Cappelli, Katia
collection PubMed
description BACKGROUND: The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders. In this study, the molecular mechanisms involved in the response to acute and strenuous exercise were investigated using peripheral blood mononuclear cells (PBMCs). RESULTS: Quantitative real-time PCR (qRT-PCR) was used to detect modifications in transcription levels of the genes for matrix metalloproteinase-1 (MMP-1) and interleukin 8 (IL-8), which were derived from previous genome-wide expression analysis. Significant up-regulation of these two genes was found in 10 horses that had completed a race of 90–120 km in a time-course experimental design. CONCLUSION: These results suggest that MMP-1 and IL-8 are both involved in the exercise-induced stress response, and this represents a starting point from which to understand the adaptive responses to this phenomenon.
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spelling pubmed-27053402009-07-03 Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses Cappelli, Katia Felicetti, Michela Capomaccio, Stefano Pieramati, Camillo Silvestrelli, Maurizio Verini-Supplizi, Andrea BMC Physiol Research Article BACKGROUND: The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders. In this study, the molecular mechanisms involved in the response to acute and strenuous exercise were investigated using peripheral blood mononuclear cells (PBMCs). RESULTS: Quantitative real-time PCR (qRT-PCR) was used to detect modifications in transcription levels of the genes for matrix metalloproteinase-1 (MMP-1) and interleukin 8 (IL-8), which were derived from previous genome-wide expression analysis. Significant up-regulation of these two genes was found in 10 horses that had completed a race of 90–120 km in a time-course experimental design. CONCLUSION: These results suggest that MMP-1 and IL-8 are both involved in the exercise-induced stress response, and this represents a starting point from which to understand the adaptive responses to this phenomenon. BioMed Central 2009-06-24 /pmc/articles/PMC2705340/ /pubmed/19552796 http://dx.doi.org/10.1186/1472-6793-9-12 Text en Copyright © 2009 Cappelli et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cappelli, Katia
Felicetti, Michela
Capomaccio, Stefano
Pieramati, Camillo
Silvestrelli, Maurizio
Verini-Supplizi, Andrea
Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
title Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
title_full Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
title_fullStr Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
title_full_unstemmed Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
title_short Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
title_sort exercise-induced up-regulation of mmp-1 and il-8 genes in endurance horses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705340/
https://www.ncbi.nlm.nih.gov/pubmed/19552796
http://dx.doi.org/10.1186/1472-6793-9-12
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