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Power analysis of QTL detection in half-sib families using selective DNA pooling

Individual loci of economic importance (QTL) can be detected by comparing the inheritance of a trait and the inheritance of loci with alleles readily identifiable by laboratory methods (genetic markers). Data on allele segregation at the individual level are costly and alternatives have been propose...

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Autores principales: Baro, Jesús Á, Carleos, Carlos, Corral, Norberto, López, Teresa, Cañón, Javier
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705406/
https://www.ncbi.nlm.nih.gov/pubmed/11403746
http://dx.doi.org/10.1186/1297-9686-33-3-231
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author Baro, Jesús Á
Carleos, Carlos
Corral, Norberto
López, Teresa
Cañón, Javier
author_facet Baro, Jesús Á
Carleos, Carlos
Corral, Norberto
López, Teresa
Cañón, Javier
author_sort Baro, Jesús Á
collection PubMed
description Individual loci of economic importance (QTL) can be detected by comparing the inheritance of a trait and the inheritance of loci with alleles readily identifiable by laboratory methods (genetic markers). Data on allele segregation at the individual level are costly and alternatives have been proposed that make use of allele frequencies among progeny, rather than individual genotypes. Among the factors that may affect the power of the set up, the most important are those intrinsic to the QTL: the additive effect of the QTL, and its dominance, and distance between markers and QTL. Other factors are relative to the choice of animals and markers, such as the frequency of the QTL and marker alleles among dams and sires. Data collection may affect the detection power through the size of half-sib families, selection rate within families, and the technical error incurred when estimating genetic frequencies. We present results for a sensitivity analysis for QTL detection using pools of DNA from selected half-sibs. Simulations showed that conclusive detection may be achieved with families of at least 500 half-sibs if sires are chosen on the criteria that most of their marker alleles are either both missing, or one is fixed, among dams.
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spelling pubmed-27054062009-07-03 Power analysis of QTL detection in half-sib families using selective DNA pooling Baro, Jesús Á Carleos, Carlos Corral, Norberto López, Teresa Cañón, Javier Genet Sel Evol Research Individual loci of economic importance (QTL) can be detected by comparing the inheritance of a trait and the inheritance of loci with alleles readily identifiable by laboratory methods (genetic markers). Data on allele segregation at the individual level are costly and alternatives have been proposed that make use of allele frequencies among progeny, rather than individual genotypes. Among the factors that may affect the power of the set up, the most important are those intrinsic to the QTL: the additive effect of the QTL, and its dominance, and distance between markers and QTL. Other factors are relative to the choice of animals and markers, such as the frequency of the QTL and marker alleles among dams and sires. Data collection may affect the detection power through the size of half-sib families, selection rate within families, and the technical error incurred when estimating genetic frequencies. We present results for a sensitivity analysis for QTL detection using pools of DNA from selected half-sibs. Simulations showed that conclusive detection may be achieved with families of at least 500 half-sibs if sires are chosen on the criteria that most of their marker alleles are either both missing, or one is fixed, among dams. BioMed Central 2001-05-15 /pmc/articles/PMC2705406/ /pubmed/11403746 http://dx.doi.org/10.1186/1297-9686-33-3-231 Text en Copyright © 2001 INRA, EDP Sciences
spellingShingle Research
Baro, Jesús Á
Carleos, Carlos
Corral, Norberto
López, Teresa
Cañón, Javier
Power analysis of QTL detection in half-sib families using selective DNA pooling
title Power analysis of QTL detection in half-sib families using selective DNA pooling
title_full Power analysis of QTL detection in half-sib families using selective DNA pooling
title_fullStr Power analysis of QTL detection in half-sib families using selective DNA pooling
title_full_unstemmed Power analysis of QTL detection in half-sib families using selective DNA pooling
title_short Power analysis of QTL detection in half-sib families using selective DNA pooling
title_sort power analysis of qtl detection in half-sib families using selective dna pooling
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705406/
https://www.ncbi.nlm.nih.gov/pubmed/11403746
http://dx.doi.org/10.1186/1297-9686-33-3-231
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