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Genetic diversity in Spanish donkey breeds using microsatellite DNA markers
Genetic diversity at 13 equine microsatellite loci was compared in five endangered Spanish donkey breeds: Andaluza, Catalana, Mallorquina, Encartaciones and Zamorano-Leonesa. All of the equine microsatellites used in this study were amplified and were polymorphic in the domestic donkey breeds with t...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2001
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705415/ https://www.ncbi.nlm.nih.gov/pubmed/11559485 http://dx.doi.org/10.1186/1297-9686-33-4-433 |
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author | Aranguren-Méndez, José Jordana, Jordi Gomez, Mariano |
author_facet | Aranguren-Méndez, José Jordana, Jordi Gomez, Mariano |
author_sort | Aranguren-Méndez, José |
collection | PubMed |
description | Genetic diversity at 13 equine microsatellite loci was compared in five endangered Spanish donkey breeds: Andaluza, Catalana, Mallorquina, Encartaciones and Zamorano-Leonesa. All of the equine microsatellites used in this study were amplified and were polymorphic in the domestic donkey breeds with the exception of HMS1, which was monomorphic, and ASB2, which failed to amplify. Allele number, frequency distributions and mean heterozygosities were very similar among the Spanish donkey breeds. The unbiased expected heterozygosity (H(E)) over all the populations varied between 0.637 and 0.684 in this study. The low G(ST )value showed that only 3.6% of the diversity was between breeds (P < 0.01). Significant deviations from Hardy-Weinberg equilibrium were shown for a number of locus-population combinations, except HMS5 that showed agreement in all analysed populations. The cumulative exclusion probability (PE) was 0.999 in each breed, suggesting that the loci would be suitable for donkey parentage testing. The constructed dendrogram from the D(A )distance matrix showed little differentiation between Spanish breeds, but great differentiation between them and the Moroccan ass and also with the horse, used as an outgroup. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in domestic donkey populations, which could also be useful for conservation plans. |
format | Text |
id | pubmed-2705415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27054152009-07-03 Genetic diversity in Spanish donkey breeds using microsatellite DNA markers Aranguren-Méndez, José Jordana, Jordi Gomez, Mariano Genet Sel Evol Research Genetic diversity at 13 equine microsatellite loci was compared in five endangered Spanish donkey breeds: Andaluza, Catalana, Mallorquina, Encartaciones and Zamorano-Leonesa. All of the equine microsatellites used in this study were amplified and were polymorphic in the domestic donkey breeds with the exception of HMS1, which was monomorphic, and ASB2, which failed to amplify. Allele number, frequency distributions and mean heterozygosities were very similar among the Spanish donkey breeds. The unbiased expected heterozygosity (H(E)) over all the populations varied between 0.637 and 0.684 in this study. The low G(ST )value showed that only 3.6% of the diversity was between breeds (P < 0.01). Significant deviations from Hardy-Weinberg equilibrium were shown for a number of locus-population combinations, except HMS5 that showed agreement in all analysed populations. The cumulative exclusion probability (PE) was 0.999 in each breed, suggesting that the loci would be suitable for donkey parentage testing. The constructed dendrogram from the D(A )distance matrix showed little differentiation between Spanish breeds, but great differentiation between them and the Moroccan ass and also with the horse, used as an outgroup. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in domestic donkey populations, which could also be useful for conservation plans. BioMed Central 2001-07-15 /pmc/articles/PMC2705415/ /pubmed/11559485 http://dx.doi.org/10.1186/1297-9686-33-4-433 Text en Copyright © 2001 INRA, EDP Sciences |
spellingShingle | Research Aranguren-Méndez, José Jordana, Jordi Gomez, Mariano Genetic diversity in Spanish donkey breeds using microsatellite DNA markers |
title | Genetic diversity in Spanish donkey breeds using microsatellite DNA markers |
title_full | Genetic diversity in Spanish donkey breeds using microsatellite DNA markers |
title_fullStr | Genetic diversity in Spanish donkey breeds using microsatellite DNA markers |
title_full_unstemmed | Genetic diversity in Spanish donkey breeds using microsatellite DNA markers |
title_short | Genetic diversity in Spanish donkey breeds using microsatellite DNA markers |
title_sort | genetic diversity in spanish donkey breeds using microsatellite dna markers |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705415/ https://www.ncbi.nlm.nih.gov/pubmed/11559485 http://dx.doi.org/10.1186/1297-9686-33-4-433 |
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