Cargando…

ChickRH6: a chicken whole-genome radiation hybrid panel

As a first step towards the development of radiation hybrid maps, we have produced a radiation hybrid panel in the chicken by fusing female embryonic diploid fibroblasts irradiated at 6 000 rads with HPRT-deficient hamster Wg3hCl(2 )cells. Due to the low retention frequency of the chicken fragments,...

Descripción completa

Detalles Bibliográficos
Autores principales: Morisson, Mireille, Lemière, Alexandre, Bosc, Sarah, Galan, Maxime, Plisson-Petit, Florence, Pinton, Philippe, Delcros, Chantal, Fève, Katia, Pitel, Frédérique, Fillon, Valérie, Yerle, Martine, Vignal, Alain
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705459/
https://www.ncbi.nlm.nih.gov/pubmed/12270108
http://dx.doi.org/10.1186/1297-9686-34-4-521
_version_ 1782169004888031232
author Morisson, Mireille
Lemière, Alexandre
Bosc, Sarah
Galan, Maxime
Plisson-Petit, Florence
Pinton, Philippe
Delcros, Chantal
Fève, Katia
Pitel, Frédérique
Fillon, Valérie
Yerle, Martine
Vignal, Alain
author_facet Morisson, Mireille
Lemière, Alexandre
Bosc, Sarah
Galan, Maxime
Plisson-Petit, Florence
Pinton, Philippe
Delcros, Chantal
Fève, Katia
Pitel, Frédérique
Fillon, Valérie
Yerle, Martine
Vignal, Alain
author_sort Morisson, Mireille
collection PubMed
description As a first step towards the development of radiation hybrid maps, we have produced a radiation hybrid panel in the chicken by fusing female embryonic diploid fibroblasts irradiated at 6 000 rads with HPRT-deficient hamster Wg3hCl(2 )cells. Due to the low retention frequency of the chicken fragments, a high number of clones was produced from which the best ones were selected. Thus, 452 fusion clones were tested for retention frequencies with a panel of 46 markers. Based on these results, 103 clones with a mean marker retention of 23.8% were selected for large scale culture to produce DNA in sufficient quantities for the genotyping of numerous markers. Retention frequency was tested again with the same 46 markers and the 90 best clones, with a final mean retention frequency of 21.9%, were selected for the final panel. This panel will be a valuable resource for fine mapping of markers and genes in the chicken, and will also help in building BAC contigs.
format Text
id pubmed-2705459
institution National Center for Biotechnology Information
language English
publishDate 2002
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-27054592009-07-03 ChickRH6: a chicken whole-genome radiation hybrid panel Morisson, Mireille Lemière, Alexandre Bosc, Sarah Galan, Maxime Plisson-Petit, Florence Pinton, Philippe Delcros, Chantal Fève, Katia Pitel, Frédérique Fillon, Valérie Yerle, Martine Vignal, Alain Genet Sel Evol Research As a first step towards the development of radiation hybrid maps, we have produced a radiation hybrid panel in the chicken by fusing female embryonic diploid fibroblasts irradiated at 6 000 rads with HPRT-deficient hamster Wg3hCl(2 )cells. Due to the low retention frequency of the chicken fragments, a high number of clones was produced from which the best ones were selected. Thus, 452 fusion clones were tested for retention frequencies with a panel of 46 markers. Based on these results, 103 clones with a mean marker retention of 23.8% were selected for large scale culture to produce DNA in sufficient quantities for the genotyping of numerous markers. Retention frequency was tested again with the same 46 markers and the 90 best clones, with a final mean retention frequency of 21.9%, were selected for the final panel. This panel will be a valuable resource for fine mapping of markers and genes in the chicken, and will also help in building BAC contigs. BioMed Central 2002-07-15 /pmc/articles/PMC2705459/ /pubmed/12270108 http://dx.doi.org/10.1186/1297-9686-34-4-521 Text en Copyright © 2002 INRA, EDP Sciences
spellingShingle Research
Morisson, Mireille
Lemière, Alexandre
Bosc, Sarah
Galan, Maxime
Plisson-Petit, Florence
Pinton, Philippe
Delcros, Chantal
Fève, Katia
Pitel, Frédérique
Fillon, Valérie
Yerle, Martine
Vignal, Alain
ChickRH6: a chicken whole-genome radiation hybrid panel
title ChickRH6: a chicken whole-genome radiation hybrid panel
title_full ChickRH6: a chicken whole-genome radiation hybrid panel
title_fullStr ChickRH6: a chicken whole-genome radiation hybrid panel
title_full_unstemmed ChickRH6: a chicken whole-genome radiation hybrid panel
title_short ChickRH6: a chicken whole-genome radiation hybrid panel
title_sort chickrh6: a chicken whole-genome radiation hybrid panel
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705459/
https://www.ncbi.nlm.nih.gov/pubmed/12270108
http://dx.doi.org/10.1186/1297-9686-34-4-521
work_keys_str_mv AT morissonmireille chickrh6achickenwholegenomeradiationhybridpanel
AT lemierealexandre chickrh6achickenwholegenomeradiationhybridpanel
AT boscsarah chickrh6achickenwholegenomeradiationhybridpanel
AT galanmaxime chickrh6achickenwholegenomeradiationhybridpanel
AT plissonpetitflorence chickrh6achickenwholegenomeradiationhybridpanel
AT pintonphilippe chickrh6achickenwholegenomeradiationhybridpanel
AT delcroschantal chickrh6achickenwholegenomeradiationhybridpanel
AT fevekatia chickrh6achickenwholegenomeradiationhybridpanel
AT pitelfrederique chickrh6achickenwholegenomeradiationhybridpanel
AT fillonvalerie chickrh6achickenwholegenomeradiationhybridpanel
AT yerlemartine chickrh6achickenwholegenomeradiationhybridpanel
AT vignalalain chickrh6achickenwholegenomeradiationhybridpanel