In Vivo Colocalisation of oskar mRNA and Trans-Acting Proteins Revealed by Quantitative Imaging of the Drosophila Oocyte

Efficient mRNA transport in eukaryotes requires highly orchestrated relationships between nuclear and cytoplasmic proteins. For oskar mRNA, the Drosophila posterior determinant, these spatio-temporal requirements remain opaque during its multi-step transport process. By in vivo covisualization of os...

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Autores principales: Mhlanga, Musa M., Bratu, Diana P., Genovesio, Auguste, Rybarska, Agata, Chenouard, Nicolas, Nehrbass, Ulf, Olivo-Marin, Jean-Christophe
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705681/
https://www.ncbi.nlm.nih.gov/pubmed/19597554
http://dx.doi.org/10.1371/journal.pone.0006241
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author Mhlanga, Musa M.
Bratu, Diana P.
Genovesio, Auguste
Rybarska, Agata
Chenouard, Nicolas
Nehrbass, Ulf
Olivo-Marin, Jean-Christophe
author_facet Mhlanga, Musa M.
Bratu, Diana P.
Genovesio, Auguste
Rybarska, Agata
Chenouard, Nicolas
Nehrbass, Ulf
Olivo-Marin, Jean-Christophe
author_sort Mhlanga, Musa M.
collection PubMed
description Efficient mRNA transport in eukaryotes requires highly orchestrated relationships between nuclear and cytoplasmic proteins. For oskar mRNA, the Drosophila posterior determinant, these spatio-temporal requirements remain opaque during its multi-step transport process. By in vivo covisualization of oskar mRNA with Staufen, its putative trafficking protein, we find oskar mRNA to be present in particles distinct from Staufen for part of its transport. oskar mRNA stably associated with Staufen near the posterior pole. We observe oskar mRNA to oligomerize as hundreds of copies forming large particles which are necessary for its long range transport and localization. We show the formation of these particles occurs in the nurse cell nucleus in an Hrp48-dependent manner. We present a more refined model of oskar mRNA transport in the Drosophila oocyte.
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spelling pubmed-27056812009-07-14 In Vivo Colocalisation of oskar mRNA and Trans-Acting Proteins Revealed by Quantitative Imaging of the Drosophila Oocyte Mhlanga, Musa M. Bratu, Diana P. Genovesio, Auguste Rybarska, Agata Chenouard, Nicolas Nehrbass, Ulf Olivo-Marin, Jean-Christophe PLoS One Research Article Efficient mRNA transport in eukaryotes requires highly orchestrated relationships between nuclear and cytoplasmic proteins. For oskar mRNA, the Drosophila posterior determinant, these spatio-temporal requirements remain opaque during its multi-step transport process. By in vivo covisualization of oskar mRNA with Staufen, its putative trafficking protein, we find oskar mRNA to be present in particles distinct from Staufen for part of its transport. oskar mRNA stably associated with Staufen near the posterior pole. We observe oskar mRNA to oligomerize as hundreds of copies forming large particles which are necessary for its long range transport and localization. We show the formation of these particles occurs in the nurse cell nucleus in an Hrp48-dependent manner. We present a more refined model of oskar mRNA transport in the Drosophila oocyte. Public Library of Science 2009-07-14 /pmc/articles/PMC2705681/ /pubmed/19597554 http://dx.doi.org/10.1371/journal.pone.0006241 Text en Mhlanga et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mhlanga, Musa M.
Bratu, Diana P.
Genovesio, Auguste
Rybarska, Agata
Chenouard, Nicolas
Nehrbass, Ulf
Olivo-Marin, Jean-Christophe
In Vivo Colocalisation of oskar mRNA and Trans-Acting Proteins Revealed by Quantitative Imaging of the Drosophila Oocyte
title In Vivo Colocalisation of oskar mRNA and Trans-Acting Proteins Revealed by Quantitative Imaging of the Drosophila Oocyte
title_full In Vivo Colocalisation of oskar mRNA and Trans-Acting Proteins Revealed by Quantitative Imaging of the Drosophila Oocyte
title_fullStr In Vivo Colocalisation of oskar mRNA and Trans-Acting Proteins Revealed by Quantitative Imaging of the Drosophila Oocyte
title_full_unstemmed In Vivo Colocalisation of oskar mRNA and Trans-Acting Proteins Revealed by Quantitative Imaging of the Drosophila Oocyte
title_short In Vivo Colocalisation of oskar mRNA and Trans-Acting Proteins Revealed by Quantitative Imaging of the Drosophila Oocyte
title_sort in vivo colocalisation of oskar mrna and trans-acting proteins revealed by quantitative imaging of the drosophila oocyte
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2705681/
https://www.ncbi.nlm.nih.gov/pubmed/19597554
http://dx.doi.org/10.1371/journal.pone.0006241
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