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Use of an EZ-Tn5-Based Random Mutagenesis System to Identify a Novel Toxin Regulatory Locus in Clostridium perfringens Strain 13
BACKGROUND: Although useful for probing bacterial pathogenesis and physiology, current random mutagenesis systems suffer limitations for studying the toxin-producing bacterium Clostridium perfringens. METHODOLOGY/PRINCIPAL FINDINGS: An EZ-Tn5-based random mutagenesis approach was developed for use i...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706048/ https://www.ncbi.nlm.nih.gov/pubmed/19597556 http://dx.doi.org/10.1371/journal.pone.0006232 |