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Use of an EZ-Tn5-Based Random Mutagenesis System to Identify a Novel Toxin Regulatory Locus in Clostridium perfringens Strain 13

BACKGROUND: Although useful for probing bacterial pathogenesis and physiology, current random mutagenesis systems suffer limitations for studying the toxin-producing bacterium Clostridium perfringens. METHODOLOGY/PRINCIPAL FINDINGS: An EZ-Tn5-based random mutagenesis approach was developed for use i...

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Detalles Bibliográficos
Autores principales:	Vidal, Jorge E., Chen, Jianming, Li, Jihong, McClane, Bruce A.
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2009
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706048/ https://www.ncbi.nlm.nih.gov/pubmed/19597556 http://dx.doi.org/10.1371/journal.pone.0006232

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