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Generation and characterization of human insulin-releasing cell lines
BACKGROUND: The in vitro culture of insulinomas provides an attractive tool to study cell proliferation and insulin synthesis and secretion. However, only a few human beta cell lines have been described, with long-term passage resulting in loss of insulin secretion. Therefore, we set out to establis...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706802/ https://www.ncbi.nlm.nih.gov/pubmed/19545371 http://dx.doi.org/10.1186/1471-2121-10-49 |
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author | Labriola, Leticia Peters, Maria G Krogh, Karin Stigliano, Iván Terra, Letícia F Buchanan, Cecilia Machado, Marcel CC Joffé, Elisa Bal de Kier Puricelli, Lydia Sogayar, Mari C |
author_facet | Labriola, Leticia Peters, Maria G Krogh, Karin Stigliano, Iván Terra, Letícia F Buchanan, Cecilia Machado, Marcel CC Joffé, Elisa Bal de Kier Puricelli, Lydia Sogayar, Mari C |
author_sort | Labriola, Leticia |
collection | PubMed |
description | BACKGROUND: The in vitro culture of insulinomas provides an attractive tool to study cell proliferation and insulin synthesis and secretion. However, only a few human beta cell lines have been described, with long-term passage resulting in loss of insulin secretion. Therefore, we set out to establish and characterize human insulin-releasing cell lines. RESULTS: We generated ex-vivo primary cultures from two independent human insulinomas and from a human nesidioblastosis, all of which were cultured up to passage number 20. All cell lines secreted human insulin and C-peptide. These cell lines expressed neuroendocrine and islets markers, confirming the expression profile found in the biopsies. Although all beta cell lineages survived an anchorage independent culture, none of them were able to invade an extracellular matrix substrate. CONCLUSION: We have established three human insulin-releasing cell lines which maintain antigenic characteristics and insulin secretion profiles of the original tumors. These cell lines represent valuable tools for the study of molecular events underlying beta cell function and dysfunction. |
format | Text |
id | pubmed-2706802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27068022009-07-08 Generation and characterization of human insulin-releasing cell lines Labriola, Leticia Peters, Maria G Krogh, Karin Stigliano, Iván Terra, Letícia F Buchanan, Cecilia Machado, Marcel CC Joffé, Elisa Bal de Kier Puricelli, Lydia Sogayar, Mari C BMC Cell Biol Research Article BACKGROUND: The in vitro culture of insulinomas provides an attractive tool to study cell proliferation and insulin synthesis and secretion. However, only a few human beta cell lines have been described, with long-term passage resulting in loss of insulin secretion. Therefore, we set out to establish and characterize human insulin-releasing cell lines. RESULTS: We generated ex-vivo primary cultures from two independent human insulinomas and from a human nesidioblastosis, all of which were cultured up to passage number 20. All cell lines secreted human insulin and C-peptide. These cell lines expressed neuroendocrine and islets markers, confirming the expression profile found in the biopsies. Although all beta cell lineages survived an anchorage independent culture, none of them were able to invade an extracellular matrix substrate. CONCLUSION: We have established three human insulin-releasing cell lines which maintain antigenic characteristics and insulin secretion profiles of the original tumors. These cell lines represent valuable tools for the study of molecular events underlying beta cell function and dysfunction. BioMed Central 2009-06-19 /pmc/articles/PMC2706802/ /pubmed/19545371 http://dx.doi.org/10.1186/1471-2121-10-49 Text en Copyright © 2009 Labriola et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Labriola, Leticia Peters, Maria G Krogh, Karin Stigliano, Iván Terra, Letícia F Buchanan, Cecilia Machado, Marcel CC Joffé, Elisa Bal de Kier Puricelli, Lydia Sogayar, Mari C Generation and characterization of human insulin-releasing cell lines |
title | Generation and characterization of human insulin-releasing cell lines |
title_full | Generation and characterization of human insulin-releasing cell lines |
title_fullStr | Generation and characterization of human insulin-releasing cell lines |
title_full_unstemmed | Generation and characterization of human insulin-releasing cell lines |
title_short | Generation and characterization of human insulin-releasing cell lines |
title_sort | generation and characterization of human insulin-releasing cell lines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706802/ https://www.ncbi.nlm.nih.gov/pubmed/19545371 http://dx.doi.org/10.1186/1471-2121-10-49 |
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