Cargando…

Cytogenetic mapping of 25 goat mammary gland Expressed Sequence Tags (ESTs)

Today, there is a shift towards a positional candidate approach in the molecular identification of genes. This study reports on an Expressed Sequence Tags (ESTs) mapping initiative in goats, based on sequence information gathered from a previous mammary gland cDNA systematic sequencing project. A to...

Descripción completa

Detalles Bibliográficos
Autores principales: Le Provost, Fabienne, Schibler, Laurent, Oustry-Vaiman, Anne, Martin, Patrice, Cribiu, Edmond P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706890/
https://www.ncbi.nlm.nih.gov/pubmed/14736395
http://dx.doi.org/10.1186/1297-9686-32-3-311
_version_ 1782169110846636032
author Le Provost, Fabienne
Schibler, Laurent
Oustry-Vaiman, Anne
Martin, Patrice
Cribiu, Edmond P
author_facet Le Provost, Fabienne
Schibler, Laurent
Oustry-Vaiman, Anne
Martin, Patrice
Cribiu, Edmond P
author_sort Le Provost, Fabienne
collection PubMed
description Today, there is a shift towards a positional candidate approach in the molecular identification of genes. This study reports on an Expressed Sequence Tags (ESTs) mapping initiative in goats, based on sequence information gathered from a previous mammary gland cDNA systematic sequencing project. A total of 25 novel genes was localised cytogenetically on 16 goat chromosomes. Six of these ESTs were found to map to cattle milk QTL regions. These results made it possible to assess the use of ESTs as a shortcut to the molecular identification of some QTLs and as a valuable tool for comparative mapping.
format Text
id pubmed-2706890
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-27068902009-07-08 Cytogenetic mapping of 25 goat mammary gland Expressed Sequence Tags (ESTs) Le Provost, Fabienne Schibler, Laurent Oustry-Vaiman, Anne Martin, Patrice Cribiu, Edmond P Genet Sel Evol Research Today, there is a shift towards a positional candidate approach in the molecular identification of genes. This study reports on an Expressed Sequence Tags (ESTs) mapping initiative in goats, based on sequence information gathered from a previous mammary gland cDNA systematic sequencing project. A total of 25 novel genes was localised cytogenetically on 16 goat chromosomes. Six of these ESTs were found to map to cattle milk QTL regions. These results made it possible to assess the use of ESTs as a shortcut to the molecular identification of some QTLs and as a valuable tool for comparative mapping. BioMed Central 2000-05-15 /pmc/articles/PMC2706890/ /pubmed/14736395 http://dx.doi.org/10.1186/1297-9686-32-3-311 Text en Copyright © 2000 INRA, EDP Sciences
spellingShingle Research
Le Provost, Fabienne
Schibler, Laurent
Oustry-Vaiman, Anne
Martin, Patrice
Cribiu, Edmond P
Cytogenetic mapping of 25 goat mammary gland Expressed Sequence Tags (ESTs)
title Cytogenetic mapping of 25 goat mammary gland Expressed Sequence Tags (ESTs)
title_full Cytogenetic mapping of 25 goat mammary gland Expressed Sequence Tags (ESTs)
title_fullStr Cytogenetic mapping of 25 goat mammary gland Expressed Sequence Tags (ESTs)
title_full_unstemmed Cytogenetic mapping of 25 goat mammary gland Expressed Sequence Tags (ESTs)
title_short Cytogenetic mapping of 25 goat mammary gland Expressed Sequence Tags (ESTs)
title_sort cytogenetic mapping of 25 goat mammary gland expressed sequence tags (ests)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706890/
https://www.ncbi.nlm.nih.gov/pubmed/14736395
http://dx.doi.org/10.1186/1297-9686-32-3-311
work_keys_str_mv AT leprovostfabienne cytogeneticmappingof25goatmammaryglandexpressedsequencetagsests
AT schiblerlaurent cytogeneticmappingof25goatmammaryglandexpressedsequencetagsests
AT oustryvaimananne cytogeneticmappingof25goatmammaryglandexpressedsequencetagsests
AT martinpatrice cytogeneticmappingof25goatmammaryglandexpressedsequencetagsests
AT cribiuedmondp cytogeneticmappingof25goatmammaryglandexpressedsequencetagsests