A high-density linkage map of the RN region in pigs

The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP marker...

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Detalles Bibliográficos
Autores principales: Looft, Christian, Milan, Denis, Jeon, Jin-Tae, Paul, Sven, Reinsch, Norbert, Rogel-Gaillard, Claire, Rey, Virginie, Amarger, Valérie, Robic, Annie, Kalm, Ernst, Chardon, Patrick, Andersson, Leif
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2000
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706891/
https://www.ncbi.nlm.nih.gov/pubmed/14736396
http://dx.doi.org/10.1186/1297-9686-32-3-321
Descripción
Sumario:The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene.

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