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A high-density linkage map of the RN region in pigs
The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP marker...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2000
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706891/ https://www.ncbi.nlm.nih.gov/pubmed/14736396 http://dx.doi.org/10.1186/1297-9686-32-3-321 |
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author | Looft, Christian Milan, Denis Jeon, Jin-Tae Paul, Sven Reinsch, Norbert Rogel-Gaillard, Claire Rey, Virginie Amarger, Valérie Robic, Annie Kalm, Ernst Chardon, Patrick Andersson, Leif |
author_facet | Looft, Christian Milan, Denis Jeon, Jin-Tae Paul, Sven Reinsch, Norbert Rogel-Gaillard, Claire Rey, Virginie Amarger, Valérie Robic, Annie Kalm, Ernst Chardon, Patrick Andersson, Leif |
author_sort | Looft, Christian |
collection | PubMed |
description | The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene. |
format | Text |
id | pubmed-2706891 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27068912009-07-08 A high-density linkage map of the RN region in pigs Looft, Christian Milan, Denis Jeon, Jin-Tae Paul, Sven Reinsch, Norbert Rogel-Gaillard, Claire Rey, Virginie Amarger, Valérie Robic, Annie Kalm, Ernst Chardon, Patrick Andersson, Leif Genet Sel Evol Research The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene. BioMed Central 2000-05-15 /pmc/articles/PMC2706891/ /pubmed/14736396 http://dx.doi.org/10.1186/1297-9686-32-3-321 Text en Copyright © 2000 INRA, EDP Sciences |
spellingShingle | Research Looft, Christian Milan, Denis Jeon, Jin-Tae Paul, Sven Reinsch, Norbert Rogel-Gaillard, Claire Rey, Virginie Amarger, Valérie Robic, Annie Kalm, Ernst Chardon, Patrick Andersson, Leif A high-density linkage map of the RN region in pigs |
title | A high-density linkage map of the RN region in pigs |
title_full | A high-density linkage map of the RN region in pigs |
title_fullStr | A high-density linkage map of the RN region in pigs |
title_full_unstemmed | A high-density linkage map of the RN region in pigs |
title_short | A high-density linkage map of the RN region in pigs |
title_sort | high-density linkage map of the rn region in pigs |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2706891/ https://www.ncbi.nlm.nih.gov/pubmed/14736396 http://dx.doi.org/10.1186/1297-9686-32-3-321 |
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